title page
Contents
Abbreviations 8
ABSTRACT 9
Introduction 12
Material and methods 19
1. Retrograde labeling of spinally projecting PVN neurons 20
2. Slice preparation and maintenance 21
3. Electrophysiological recording 21
4. Cell identification 22
5. Recording and analysis of inhibitory postsynaptic currents 23
6. Drug application 24
7. Statistical analysis 24
Results 26
Discussion 46
Conclusions 54
References 56
초록 69
Table 1. Electrophysiological properties of DiI-labeled and DiI-unlabeled type II PVN nerons (mean±SEM) 32
List of Flgure
Fig. 1. Identification of a retrogradely labeled spinally projecting paraventricular nucleus(PVN) neurons. 33
Fig. 2. Electrophysiological classification of spinally projecting paraventricular neurons by a depolarizing current steps with a pre-pulse. 34
Fig. 3. Typical example of spontaneous inhibitory postsynaptic current (sIPSC) from DiI-labeled PVN neurons. 35
Fig. 4. Effect of 5-HT on spontaneous inhibitory postsynaptic current (sIPSC) of Dil-labeled PVN neurons. 36
Fig. 5. Effect of 5-HT on sIPSC of spinally projecting paraventricular neurons. 37
Fig. 6. Concentration dependence of the effects of 5-HT on sIpSC frequency in DiI-labeled PVN neurons. 38
Fig. 7. Effect of 5-HT on sIPSC before and after application of CNQX (6-cyano-7-nitroquinoxaline-2,3-dione) in DiI-labeled PVN neurons. 39
Fig. 8. 8-OH-DPAT mimics the inhibitory effect of 5-HT on sIPSCs of DiI-labeled PVN neurons. 40
Fig. 9. Concentration-dependent of sIPSC frequency by 5-HT in DiI-unlabled type II PVN neurons. 41
Fig. 10. 5-HT response pattern of the DiI-labeled and DiI-unlabled Type II PVN neurons. 42
Fig. 11. Stimulatory effect of 5-HT on the firing activity of DiI-labeled PVN neurons. 43
Fig. 12. Apparent inconsistency of 5-HT effect on sIPSC and firing rate in 1 of 9 DiI-labeled PVN neuron tested. 44
Fig. 13. 8-OH-DPAT mimics the stimulatory effect of 5-HT on the firing activity of DiI-labeled PVN neurons. 45