표제지
초록
목차
서론 8
연구 목적 10
재료와 방법 11
동물과 수술 11
고정액의 관류 16
Cresyl violet 염색 17
광학현미경적 검사 17
면역조직화학염색 19
자료 분석과 통계 20
결과 21
1. 전뇌허혈을 유발하기 위한 예비실험 21
2. 일시적인 전뇌허혈 후 해마의 뇌세포사 22
3. Aminoguanidine의 투여가 해마의 피라밋세포사에 미치는 영향 31
4. GFAP 면역염색 34
5. CD11b 면역염색 39
6. iNOS 면역염색 44
7. Nitrotyrosine 면역염색 46
8. Bax 면역염색 49
고찰 53
결론 66
참고문헌 67
Abstract 73
Fig. 1-1 and 1-2. Rats were anesthetized and placed in the supine position. A. 2.5-cm midline skin incision was made on the neck, and with the aid of a dissection microscope, the subcutaneous connective tissue and muscles were gently retracted to expose the trachea and thyroid (1, 2). … (이미지참조) 13
Fig. 2. Viable pyramidal cells were bilaterally counted per high-power field (x400) in two and on areas in CAl and SLu (Stratum lucidem of CA3) of the hippocampus (rectangular) at the level of the coronal section, respectively. … (이미지참조) 18
Fig. 3-1. Representative photographs of the pyramidal neurons in the CA1 subfield of rats suffered from transient ischemia. … (이미지참조) 23
Fig. 3-2. Representative photographs of the pyramidal neurons in the CA1 subfield of rats suffered from transient ischemia. … (이미지참조) 24
Fig. 4. Representative phohtographs of cells (probably microglial cells and macrophages) in Stratum radiatum of the CA1 subfield of rats suffered from transient ischemia. … (이미지참조) 25
Fig. 5. Representative photographs of the pyramidal neurons in SLu (Stratum lucidem) of CA3 subfield of rats suffered from transient ischemia. … (이미지참조) 26
Fig. 6. The number of neurons in the hippocampal CA1 region. Neurons were counted per high-power field (400x) in the CA1 region. … (이미지참조) 27
Fig. 7. The number of neurons in SLu (Stratum lucidem) of hippocampal CA3. Neurons were counted per high-power field(400x) in the SLu region. … (이미지참조) 28
Fig. 8. The number of neurons in CA1 and SLu (Stratum lucidem) of CA3 of the hippocampus. Neurons were counted per high-power field(400x) in CA1 and SLu region. … (이미지참조) 29
Fig. 9. The effect of aminoguanidine (AG) on the survival of the hippocampal pyramidal neurons after 1, 3 and 7 days of reperfusion. … (이미지참조) 32
Fig. 10. The effect of aminoguanidine (AG) on the survival of the hippocampal pyramidal neurons after 1, 3 and 7 days of reperfusion. … (이미지참조) 33
Fig. 11-1, Fig.11-2. Representative photographs of the pyramidal neurons in the CA1 subfield of rats suffered from transient ischemia. … (이미지참조) 35
Fig. 12. High-power view of GFAP-positive astrocytes in CA1 regions of the hippocampus. … (이미지참조) 38
Fig. 13-1, 13-2. Representative photographs of the pyramidal neurosn in the CA1 subfield of rats suffered from transient ischema. … (이미지참조) 40
Fig. 14. High-power view of CD11b-positive astrocytes in CA1 regions of the hippocampus. … (이미지참조) 43
Fig. 15. iNOS-positive neuronal cells are seen in CA1 of the hippocampus. C3, control groups of transient global ischemia with recovery for 3 days; C3L, low-power view; C3H, high-power view. 45
Fig. 16. Representative photographs of the pyramidal neurons in the CA1 subfield of rats suffered from transient ischemia. … (이미지참조) 47
Fig. 17. Nitrotyrosine-positive cells are seen in CA1 of the hippocampus. C5 and C7, control groups of transient global ischemia with recovery for 5 and 7 days respectively. 48
Fig. 18. Representative photographs of the pyramidal neurons in the CA1 subfield of rats suffered from transient ischemia. … (이미지참조) 50
Fig. 19. High-power view of Bax-positive cells in CA1 regions of the hippocampus. C3, C5 and C7, control groups of transient global ischemia with recovery for 3, 5 and 7 days respectively. 52
Fig. 20. Instruments used in 4-Vessel occlusion in rats. 65