표제지

목차

Abbreviations 8

Ⅰ. 서론 9

Ⅱ. 연구사 12

Ⅲ. 재료 및 방법 15

1. 사양실험 및 육질분석 15

1) 공시동물과 시험사료제조분석 15

2) 육질분석 15

2. 유전자 발현 분석 19

1) 발효사료급여 돼지 특이유전자 탐색 19

2) 선발유전자의 발현양상 분석 23

Ⅲ. 결과 및 고찰 26

1. DEG분석을 통한 유전자 탐색 26

2. Sequencing 결과 29

3. Nebulin과 Titin 유전자 발현 분석 32

4. Nebulin과 Titin과 경제형질의 연관성 34

Ⅳ. 참고문헌 40

Abstract 46

감사의 글 48

Table 1. Formula Rateand Prices of Experimental Fermented Diet 17

Table 2. Feeding Period 18

Table 3. Sequnces of Primer 24

Table 4. Identification of Differentially Expressed Genes Between Formula Feed and Fermented Feedstuffs 31

Table 5. The carcass characteristics in pig steers 39

Fig 1.Genefishing DEG.PCR results for identifying differntially expressed genes between Formula Feed and Fermented Feedstuffs using arbitrary annealing primers(ACP1 to ACP10). lane M : 100bp size marker, T : Fermented Feedstuffs, C : Formula Feed. The differentially expressed... 27

Fig 2. Gene expression analysis of DEG . Gene expression analysis of DEG between Fermented Feedstuffs and Formula Feed by RT-PCR. The amplified PCR products were separated on a 2.0%a agarose gel and stained with ethidiumbromide. The spots indicated ifferentially expressed... 28

Fig 3. The expression pattens of higly expressed gene in Fermented Feedstuff feeding pig using quantitative real-time PCR. The gene expression levels of each gene were nomalized to the expression level of Nebulin(A) and Titin(B) (p〈0.05) 33