표제지
목차
Ⅰ. 서론 11
Ⅱ. 연구사 14
Ⅲ. 재료 및 방법 21
1. 실험 재료 21
1.1. 공시재료 21
1.2. 시약 및 기기 22
1.3. 발효 두충의 제조 22
1.4. 두충추출물의 조제 및 용매분획 23
2. 실험방법 25
2.1. 항 고혈압 활성 25
2.2. 항산화활성 27
2.3. 면역활성(Immuno-activity)측정 30
Ⅳ. 결과 및 고찰 32
1. 항고혈압 활성 32
2. 항산화활성 36
2.1. DPPH free radical scavenging activity 36
2.3. ABTS cation redical scavenging activity 40
2.3. FRAP (Feffic reducing antioxidant power) assay 45
3. 면역활성 48
Ⅴ. 결론 57
1. 항고혈압 활성시험 57
2. 항산화활성 시험 57
3. 면역활성시험 (Immune activity test) 58
참고문헌 59
Abstract 64
감사의 글 67
Table 1. Angiotensin converting enzyme (ACE) ingibition effect of solvent fraction isolated from nature Eucommia ulmoides Oliver 34
Table 2. Angiotensin converting enzyme (ACE) inhibition effect of solvent fractions isolated from fermented Ducommia ulmoides Oliver 34
Table 3. DPPH free radical scavenging activities of solvent fraction from nature Eucommia ulmoides Oliver 37
Table 4. DPPH free radical scavenging activities of solvent fraction from Fermented Eucommia ulmoides Oliver 39
Table 5. ABTS cation radical scavenging activities of solvent fraction from nature Eucommia ulmoides Oliver 41
Table 6. ABTS cation radical scavenging activities of solvent fraction from fermented Ducommia ulmoides Oliver 43
Table 7. Reducing power of solvent fraction from natured Cucommia ulmoides Oliver by FRAP assay 45
Table 8. Reducing power of solvent fraction from fermented Eucommia ulmoides Oliver by FRAP assay 47
Table 9. Accumulated NO₂ contents(㎛) in media for RAW264.7macrophage with LPS treated solvent fractions from nature Eucommia ulmoides Oliver(이미지참조) 49
Table 10. Accumulated NO₂ contents(㎛)in media for RAW264.7 macrophage with LPS treated solvent fractions from fermented Eucommia ulmoides Oliver(이미지참조) 51
Table 11. Accumulated NO₂contents (㎛) in media for RAW264.7 macrophage without LPS treated solvent fraction from nature Ducommia ulmoides Oliver(이미지참조) 53
Table 12. Accumulatedm NO₂contents(㎛) in media for RAW564.7 macrophage without LPS treated solvent fraction from fermented Eucommia ulmoides Oliver(이미지참조) 54
Figure 1. Produre of solvent fractionation of extracts from nature and fermented Eucommia ulmoides Oliver. 24
Figure 2. Procedure of anti-hypertension activity test by angiotension converting enzyme (ACE) inhibition 26
Figure 3. Principle of anti-hypertension activity test by renin-angiotensin system 27
Figure 4. Comparison of angiotensin converting enzyme (ACE) inhibition effects of nature and fermented Eucommia ulmoides Oliver at treated concentration of 1 mg/ml. 35
Figure 5. Comparison of angiotensin converting enzyme (ACE) inhibition effect of nature and fermented Eucommia ulmoides Oliver at treated concentration of 0.5 mg/ml. 35
Figure 6. Comparison of angiotensin converting enzyme (ACE) inhibition effects of nature and fermented Eucommia ulmoides Oliver at treated concentration of 0.1 mg/ml. 36
Figure 7. DPPH free radical scavenging activities of solvent fraction from nature Eucommia ulmoides Oliver 38
Figure 8. DPPH free radical scavenging activities of solvent fraction from Fermented Eucommia ulmoides Oliver 39
Figure 9. Comparisons of DPPH free radical scavenging activities of nature and fermented Ducommia ulmoides Oliver at treated concentration of 100 ㎍/ml 40
Figure 10. ABTS cation radical scavenging activities of solvent fraction from nature Ducommia ulmoides Oliver 42
Figure 11. ABTS cation radical scavenging activites of solvent fraction from fermented Eucommia ulmoides Oliver 43
Figure 12. Comparison of ABTS cation radical scavenging activities of nature and fermented Eucommia ulmoides Oliver at treated concentration of 100 ㎍/ml 44
Figure 13. Reducing power of solvent fraction from nature Eucommia ulmoides Oliver by FRAP assay 46
Figure 14. Reducing power of solvent fractions from fermented Eucommia ulmoides Oliver by FRAP assay 47
Figure 15. Comparison of reducing power of nature and fermented Eucommia ulmoides Oliver at treated concentration of 100 ㎍/㎖ 48
Figure 16. Accumulated NO₂ contentes (㎛)in media for RAW264.7 macrophage with LPS treated solvent fractions from nature Eucommia ulmoides Oliver(이미지참조) 50
Figure 17. Accumulated NO₂contents (㎛) in media for RAW264.7 macrophage with LPS treated solvent fractions from fermented Eucommia ulmoides Oliver(이미지참조) 51
Figure 18. Comparison of accumulated NO₂contents in the cultured media for RAW264.7 with LPS at 500㎍/ml of nature and fermented Eucommia ulmoides Oliver 52
Figure 19. Accumulated NO₂contents(㎛)in media for RAW264.7 macrophage without LPS treated solvent fraction from nature Eucommia ulmoides Oliver(이미지참조) 53
Figure 20. Accumulated NO₂contents(㎛) in media for RAW264.7 macrophage without LPS treated solvent fractions from fermented Eucommia ulmoides Oliver(이미지참조) 55
Figure 21. Comparison of accumulataed NO₂contents in the cultured media for RAW264.7 without LPS at 500㎍/ml of nature and fermented Eucommia ulmoides Oliver(이미지참조) 56
Photo 1. The pictures of Ducommia ulmodes Oliver used this research. 21