Title Page
Contents
초록 5
Abstract 6
1. Introduction 7
2. Materials and Methods 10
2.1. Materials 10
2.2. Methods 10
2.2. Synthesis of pullulan acetate pheophorbide a (PAP) conjugate 11
2.3. Procedure of PAP-Gem HCl loaded silicone film 11
2.4. In vitro release test of PAP loaded silicone film 12
2.5. In vitro singlet oxygen generation (SOG) test 12
2.6. In vitro cancer cell viability 13
2.7. In vivo tumor growth inhibition assay of PAP loaded silicone film 14
2.8. In vivo tumor growth inhibition assay of PGS film 14
3. Results & Discussion 16
3.1. Synthesis of pullulan acetate pheophorbide a (PAP) conjugate and process of film production 17
3.2. In vitro release test of PAP loaded silicone film 19
3.3. In vitro singlet oxygen generation (SOG) test 20
3.4. In vitro cancer cell viability 22
3.5. In vivo tumor growth inhibition assay of PAP loaded silicone film 23
3.6. In vivo tumor growth inhibition assay of PGS film 23
4. Conclusion 25
5. References 26