표제지
목차
I. 서론 9
II. 연구 내용 및 방법 13
1. 실험재료 13
가. 재료 13
나. 시약 및 실험기기 13
2. 실험방법 15
가. 패 추출물 제조 15
나. 총 폴리페놀 함량 15
다. 패 추출물의 항산화활성 측정 16
라. 패 추출물의 조골세포 활성 검색 17
마. 파골세포 활성 검색 20
바. 동물 실험 22
3. 통계 처리 27
III. 실험결과 및 고찰 28
1. 패 추출물 수득율 및 총 폴리페놀 함량 측정 28
2. 패 추출물의 항산화 활성 측정 29
1) 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical 소거능 측정 29
2) Superoxide dismutase (SOD) 유사활성 측정 31
3. 패 추출물의 조골세포 활성 검색 33
1) MC3T3-E1 세포의 성장률 33
2) 조골세포의 증식 유도 검색 35
3) 패 추출물의 alkaline phosphatase(ALP) 활성 효과 37
4) 조골세포의 collagen 합성에 미치는 영향 39
5) ROS (Reactive oxygen species) 생성에 대한 영향 41
4. 패 추출물의 파골세포 활성 검색 43
1) 파골세포의 성장억제 효과 검색 43
2) 파골세포의 TRAP 활성 검색 45
5. 패 추출물이 갱년기 장애를 유발한 흰 쥐의 지질 변화에 미치는 영향 47
1) 실험동물의 체중 증가량, 식이효율 및 장기중량 47
2) 패의 혈청 효소활성에 미치는 영향 51
3) 패의 항 혈전 활성 54
6. 패 추출물이 골 조직의 collagen 합성에 미치는 영향 59
1) 골 조직 중의 콜라겐 함량 59
IV. 요약 62
참고문헌 65
ABSTRACT 79
Table 1. Extraction conditions of Ishige okamurae 15
Table 2. Experimental design of animals 23
Table 3. Yield and polyphenol concentration of water and ethanol extracts of Ishige okamurae 28
Table 4. Body weight gain, food intake and food efficiency ratio of rats supplemented Ishige okamurae ethanol extracts for 7 weeks 49
Table 5. Effect of Ishige okamurae on weight of liver, heart, lung, spleen, adrenal, kidney and uterus in ovariectomized rats 50
Table 6. Effect of Ishige okamurae ethanol extracts on serum glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and alkaline phosphatase (ALP) activities in ovariectomized rats 53
Table 7. Effect of Ishige okamurae ethanol extracts on serum total-cholesterol, trigylceride, high density lipoprotein cholesterol and low density lipoprotein cholesterol, atherosclerotic index, cardiac risk factor in ovariectomized rats 55
Table 8. Effect of Ishige okamurae ethanol extracts against collagen induced platelet aggregation 58
Table 9. Effect of Ishige okamurae ethanol extracts on collagen content in cartilage, bone of ovariectomized rats 61
Fig. 1. Determination of hydroxyproline. 26
Fig. 2. Scavenging activity of DPPH radicals of various solvent fractions of Ishige okamurae. 30
Fig. 3. SOD-like activity of various solvent fractions of Ishige okamurae. 32
Fig. 4. Growth of MC3T3-E1 osteoblastic cell. 34
Fig. 5. Effects of hot water and ethanol extracts of Ishige okamurae on the Proliferation of the MC3T3-E1 osteoblastic cells by the MTT assay. MC3T3-E1 cells were cultured with vehicles or various concentrations of... 36
Fig. 6. Effects of hot water and ethanol extracts of Ishige okamurae on the alkaline phosphatase activities of the MC3T3-E1 osteoblastic cell during the differentiation.... 38
Fig. 7. Effects of hot water and ethanol extracts of Ishige okamurae on collagen content in bone of the MC3T3-E1 osteoblastic cells. 40
Fig. 8. Effects of hot water and ethanol extracts of Ishige okamurae on H₂O₂-induced reactive oxygen species (ROS) production in MC3T3-E1 cells.... 42
Fig. 9. Effects of hot water and ethanol extracts of Ishige okamurae on the viabillity of RAW 264.7 osteoclastic cell. 44
Fig. 10. TRAP activity of hot water and ethanol extracts of Ishige okamurae on osteoclast diffentiation of RAW 264.7-derived cells. 46