표제지

목차

Abbreviation (약기호표) 10

국문요약 11

제1장 서론 13

1.1. 오메가-3, 6 다가불포화지방산 13

1.2. 오메가-3, 6 다가불포화지방산 분석 18

1.3. 연구 목적 19

제2장 실험 20

2.1. 분석대상물질 및 시약 20

2.2. 표준용액 제조 22

2.3. 기체크로마토그래피-질량분석법 조건 23

2.4. 혈청시료 전처리 25

2.4.1. 고체상 추출법 최적화 25

2.4.2. 화학적 유도체화 최적화 26

2.5. 유효성검증 (Method validation)[내용누락;p.17] 28

2.6. 혈청 시료 수집 30

2.7. 통계분석 31

제3장 결과 및 고찰 32

3.1. 기체크로마토그래피-질량분석법 32

3.2. 다가불포화지방산 분석방법 최적화 40

3.2.1. 혈청 시료 준비 40

3.2.2. 고체상 추출법 최적화 41

3.2.3. 화학적 유도체화 최적화 43

3.3. 분석방법 유효성 검증 48

3.4. 고콜레스테롤혈증 토끼 혈청 시료 분석 51

제4장 결론 55

참고 문헌 57

ABSTRACT 59

Table 1. The GC-MS conditions for analysis of polyunsaturated fatty acids 24

Table 2. Calibration parameters for analysis of polyunsaturated fatty acids in serum analysis[내용누락;p.17] 29

Table 3. The GC-MS parameters for polyunsaturated fatty acids 37

Table 4. Characteristic ions obtained from different derivatizations in GC-MS analysis 47

Table 5. Results of method validation in serum analysis 49

Table 6. Intra- and inter-day validation results of polyunsaturated fatty acid in serum 50

Table 7. Concentrations of polyunsaturated fatty acids in serum of groups studied 52

Fig 1. Structure of fatty acid. 15

Fig 2. Types of fatty acids. 16

Fig 3. Metabolic pathways of omega -3, 6 fatty acids. 17

Fig 4. Chemical structure of analytes studied. 21

Fig 5. Comparison of chromatographic separation between two different columns tested. 34

Fig 6. Trimethylsilylation of fatty acids. 35

Fig 7. A total ion chromatogram of polyunsaturated fatty acids analyzed. 36

Fig 8. Mass spectra of polyunsaturated fatty acids; (A) LA, (B) ALA (C) EPA, (D) DHA, (E) DPA, (F) AA. 39

Fig 9. Comparative extraction efficiencies of fatty acids along with solid-phase extractions. 42

Fig 10. Comparative derivatizations for GC-MS analyses. 45

Fig 11. Relative recoveries along with different derivatizations in GC-MS analysis. 46

Fig 12. The levels of polyunsaturated fatty acids in rabbit sera obtained from four groups. 53

Fig 13. Comparison of two levels of (A) LA, (B) AA polyunsaturated fatty acids in rabbit sera obtained from four groups. 54

Scheme 1. Sample preparation procedure for serum samples. 27