Title Page
Contents
Introduction 4
MaterialsandMethods 6
Experimental animals 6
Surgeries (BCCAO/R) and drug administration 7
Tissue processing and hematoxylin-eosin staining 8
Fluoro-Jade Chistofluorescence staining 9
Immunoblotting 10
Open-field test 11
Immunohistochemistry 11
BV2 cell culture and cell viability assay 12
Enzyme-linked immunosorbent assay 13
Statistical analysis 14
Results 14
Protective effects of ATRAon pyramidal neurons in the hippocampal CA1 region in a model of transient global cerebral ischemia 14
Effects of ATRA on ischemia-induced hyperactivity in gerbils 16
Effects of ATRA on neuroinflammation 17
Discussion 19
References 24
Figures 31
Abstract 44