표제지
목차
Abstract 9
I. 서론 12
II. 본론 21
1. 실험 재료 21
2. 이론 및 측정 원리 25
3. 실험 방법 및 절차 30
III. 결과 및 고찰 32
1. 양성반응 검출률(Positive detection rate) 32
2. 계열별 검출 빈도(Detection frequency by antibiotic class) 34
3. 기간별 검출 빈도(Frequency of detection by period) 37
4. 항생 물질의 검출 요인(Antibiotic detection factors) 40
5. 유효성 검증(Verfication) 42
6. 식품 내 잔류 동물용 의약품에 대한 향후 과제 63
IV. 요약 66
V. 참고문헌 68
Table 1. Collection for overview for experimental samples 22
Table 2. Detection rate of positive and negative response in the sample examined 33
Table 3. Amount of antibiotics generated by each series 35
Table 4. Correlation between season and antibiotic usage 39
Table 5. Results of kit reactions with increasing antibiotic concentration 43
Table 6. Results of kit reactions with increasing antibiotic concentration 44
Table 7. Reaction result of kit on the addition of standard samples 45
Table 8. Comparison of detection limits of antibiotic residues test kit for... 52
Table 9. Comparison of detection limits of antibiotic residues test kit for... 53
Table 10. Comparison of detection limits of antibiotic residues test kit for... 54
Table 11. Comparison of detection limits of antibiotic residues test kit for... 55
Table 12. Comparison of detection limits of antibiotic residues test kit for... 56
Table 13. Comparison of detection limits of antibiotic residues test kit for... 57
Table 14. Comparison of detection limits of antibiotic residues test kit for... 58
Table 15. Identifiable and non-identifiable ratios within the range of MRLs 61
Table 16. List of antibiotics with large detection range compared to standard 62
Fig. 1. Molecular structure of penicillin G antibiotics, belonging to the beta-lactam... 14
Fig. 2. Molecular structure of tetracycline antibiotics, belonging to the tetracycline... 14
Fig. 3. Molecular structure of tylosin antibiotics, belonging to the macrolide... 15
Fig. 4. Molecular structure of gentamicin antibiotics, belonging to the... 15
Fig. 5. Molecular structure of sulfamethazine antibiotics, belonging to the... 16
Fig. 6. Molecular structure of enrofloxacin antibiotics, belonging to the quinolone... 16
Fig. 7. Mechanism of action against cells of antibiotics... 17
Fig. 8. Appearance of kits and reader in smart antibiotic residues system. 23
Fig. 9. Exploded view of antibiotic residues test kit and names of parts. 26
Fig. 10. Changes in gold conjugated pad when antibiotics are added. 27
Fig. 11. Test principle of immune antibody. 28
Fig. 12. Change in test(capture) line during positive and negative response. 29
Fig. 13. Experimental procedure using the smart antibiotic residues system. 31
Fig. 14. Percent distribution of antibiotics detected by positive reaction. 36
Fig. 15. Results of detection of antibiotics by year and month. 38
Fig. 16. Several reasons for the detection of antibiotic positives. 41
Fig. 17. Validation of the reader using standard strips with antibiotic concentrations... 46
Fig. 18. Validation certificate for reader through standard strip. 48
Fig. 19. AOAC Certificate for kit. 49