Morphological variation was used to explore the differences among geographically distinct populations for Macrobrachium rosenbergii (de Man 1879) in Indonesia. 180 samples were collected from three different locations, Sukabumi Regency, Aceh Regency, and Gresik Regency. The temperature data that presumed as a factor that determining morphological variation among locations also collected. Eleven morphometric parameters were examined, then size-standardized by the allometric method and the result are subjected to Discriminant Function Analysis (DFA) and dendogram using average linkage (between two groups) to create clusterization among the group. Our finding showed the Solo population was the biggest in size compare to the other and Siratu was the smallest. The DFA clustered the 180 samples into three groups (Siratu, Aceh, and Solo), Aceh and Solo are separated from each other while Siratu and Aceh also Siratu and Solo have an overlapping members. The temperature data are summed as degree days and analyzed to find a correlation with the morphological traits. Regression analysis between degree days and morphology parameters found there was a strong relation of the degree days and morphological parameters of Macrobrachium rosenbergii both for female and male population. Morphometric variation among these three group was a result of adaptation from the different geographical condition including the temperature.