Title Page
Contents
ABSTRACT 10
INTRODUCTION 13
MATERIALS AND METHODS 16
1. Breast cancer patients Tissues 16
2. Cell culture 16
3. Transfection 17
4. RNA and Protein extraction 17
5. qRT-PCR 18
6. Western Blotting 18
7. Cell viability Assay 19
8. Transwell Cell Migration Assay 19
9. Immunocytochemistry (ICC) and Microscopy 20
10. TCGA analysis 20
11. Statistical Analysis 21
RESULTS 23
1. CITED4 is decreased in Triple negative breast cancer. 23
2. Integrative analysis of RNA-seq and ChIP-seq data identify target gene by CITED4 overexpression 27
3. CITED4 overexpression increase ZBTB34 expression in TNBC. 30
4. ZBTB34 affects cell proliferation and migration in Triple negative breast cancer. 35
5. CITED4 exerts oncogenic function via ZBTB34 expression in Triple negative breast cancer. 38
6. Transcriptional regulatory mechanism of CITED4 - EP300 protein complex was involved in ZBTB34 regulation in Triple negative breast cancer. 41
DISCUSSION 45
REFERENCE 48
ABSTRACT IN KOREAN 50
Table 1. Information of qRT-PCR primer 22
Table 2. Information of Primary Antibody 22
Figure 1. Identification of downregulated CITED4 in Triple negative... 26
Figure 2. dentification of target genes regulated by CITED4... 29
Figure 3. ZBTB34 is downregulated in Triple negative breast... 34
Figure 4. Effect of ZBTB34 regulation on cell proliferation and cell... 37
Figure 5. CITED4 regulates cell migration and cell proliferation... 40
Figure 6. Role of transcriptional regulatory mechanism of CITED4-... 44