표제지
목차
Abstract 10
I. 서론 12
II. 재료 및 방법 15
1. 재료 15
2. 표준품 및 시약 19
3. 에톡시퀸과 에톡시퀸 다이머 분석방법 19
3.1. 표준원액 및 혼합표준용액 제조 19
3.2. 시료 전처리 20
3.3. 기기분석 22
3.4. 분석법 검증 방법 25
4. 통계처리 25
III. 결과 및 고찰 26
1. 분석법 검증 결과 26
1.1. 특이성 26
1.2. 직선성 30
1.3. 검출한계 및 정량한계 35
1.4. 회수율 및 정밀성 37
2. 에톡시퀸과 에톡시퀸 다이머 검출 결과 42
2.1. 국내 양식 어류에서 에톡시퀸과 에톡시퀸 다이머의 검출 현황 42
2.2. 국외 양식 어류에서 에톡시퀸과 에톡시퀸 다이머의 검출 현황 46
IV. 요약 50
V. 참고문헌 52
Table 1. Classification of aquacultured fish in Korea 16
Table 2. Classification of aquacultured fish in oversea 17
Table 3. LC-MS/MS parameters for ethoxyquin and ethoxyquin dimer 23
Table 4. MRM (Multiple reaction monitoring) condition for ethoxyquin and ethoxyquin dimer 24
Table 5. Conversion equation, R² of standard curves of ethoxyquin and ethoxyquin dimer in the samples tested 33
Table 6. LOD and LOQ of ethoxyquin and ethoxyquin dimer in the samples tested 36
Table 7. Recovery rates of standard ethoxyquin and ethoxyquin dimer in rockfish, salmon, shrimp and manila clam of intra day 38
Table 8. Recovery rates of standard ethoxyquin in rockfish, salmon, shrimp and manila clam of inter day 40
Table 9. Recovery rates of standard ethoxyquin dimer in rockfish, salmon, shrimp and manila clam of inter day 41
Table 10. Ethoxyquin and ethoxyquin dimer content for all samples by korea 44
Table 11. Residue concentration of ethoxyquin and ethoxyquin dimer in farmed fish by domestic 45
Table 12. Ethoxyquin and ethoxyquin dimer content for all samples by oversea 48
Table 13. Residue concentration of ethoxyquin and ethoxyquin dimer in farmed fish by oversea 49
Figure 1. Flow chart of analysis procedure for ethoxyquin and ethoxyquin dimer 21
Figure 2. LC-MS/MS chromatogram of (A) standard material, (B) blank, (C) rockfish, (D) salmon, (E) shrimp and (F) manila clam sample spiked... 29
Figure 3. Calibration curves of ethoxyquin in the samples tested 31
Figure 4. Calibration curves of ethoxyquin dimer in the samples tested 32