An advanced extraction method by ultrasonic extraction with applied solid phase extraction (SPE) has been
developed for the determination of simultaneous eight major ginsenosides, namely ginsenosides Rg1, Re, Rf, Rb1, Rg2, Rc,
Rb2, and Rd in the root of Panax ginseng. Four extraction methods including n-BuOH reflux extraction (Method A), 70%
EtOH reflux extraction (Method B), 50% MeOH reflux extraction with SPE (Method C), and 50% MeOH ultrasonication
with SPE clean-up process (Method D) were investigated for the determination of eight major ginsenosides. Total contents of
ginsenosides were highest by extraction of Method C as 2.408 ?0.011%. However, Method D was evaluated as relatively simpler
and more efficient method due to short extraction time, small solvent consumption and less expensive, compared to conservative
reflux method. Ginsenosides were also satisfactorily separated with good resolution and the accuracy range was
between 1.05 and 4.06% as relative standard deviation (RSD) by Method D. SPE condition and HPLC condition were further
optimized for determination of eight major ginsenosides by the ultrasonic extraction method. Conclusively, ultrasonic
extraction of 2 g sample of ginseng using ultrasonic bath and 1 loading for SPE was evaluated as proper condition for
extraction of ginseng.