To conduct a risk assessment of AFB1 intake, AFM1, which is a metabolite of AFB1 in the human and porcine
urine, was determined by competitive direct ELISA (cdELISA). The detection limit of cdELISA using anti-AFM1 antibody
and AFB1-HRP conjugate was 10 pg/mL. The recoveries of AFM1 were 117-167% after the addition of AFM1 in the human
urine in a range of 3-100 pg/mL. 165 samples (95.5%) of those obtained from 172 persons evidenced measurable levels of
urinary AFM1. The detected AFM1 ranges were 0-11.6 pg/mL and the average level of AFM1 contamination was 2.74?
1.89 pg/mL. The estimated amount of AFM1 excretion in the human urine was 3.97 ng/day and the estimated AFB1 intake
amount was 79.4 ng/day. The probable daily intake (PDI) of AFB1 by the subjects was estimated to be 1.28 ng/kg.bw/day,
which was higher than the tolerable daily intake (TDI, 0.15 ng/kg.bw/day). In the case of porcine urine, the AFM1 ranged
between 0.97-26.7 pg/mL and the average contaminated AFM1 was 10.62?.39 pg/mL. The estimated amount of AFM1 excretion
in the porcine urine was 27.6 ng/day, and the estimated AFB1 intake amount was 551 ng/day.