Poly-γ-glutamic acid (γ-PGA) was mixed natural flora of Bacillus subtilis,
contaminated from cooked soybeans. Also, it was performed to find out the antiallergic activity by using NC/
Nga mice, in vitro. The γ-PGA (PGA-HM : PGA-high molecular weight), Molecular weight 300 kDa, was
decomposed and made PGA-LM (PGA-low molecular weight) which has molecular weight below 30 kDa by
sonication. Therefore, it was same result between PGA-HM and PGA-LM, and reported PGA-LM as basic
result. We found that PGA-LM contains antiallergic efficacy that inhibit B cells and Th2 cells activation from
isolated CD4+ T cells in NC/Nga atopic dermatitis model mice, and not show a cytotoxicity in the hFCs. To
investigate the effects of these PGA-LM in vitro, isolation of splenic B cell and CD4+ T cells in atopic dermatitis
mice were used. To elucidate the role of PGA-LM in anti-CD40+ interleukin-4 (IL-4)-mediated B-cell
activation, showed that the capacity of B cells to expression IL-1β, IL-6, and TNF-α mRNA down-regulated,
and IL-10 mRNA up-regulation by PGA-LM treatment, but it had no effect on TGF-β expression. In addition
to CD4+IFN-γ+ and CD4+CD25+foxp3+, the functions of PGA-LM in the development of the CD4+
CD25+foxp3+ and CD4+IFN-γ+ cells, the phenotype and functions of PGA-LM induced CD4+CD25+
foxp3+, and CD4+IFN-γ+ cells in CD4+ T cells. These results suggested that PGA-LM could change cytokine
production and generate CD4+CD25+foxp3+ Tregs in NC/Nga mice, and may be effective for immunotherapy
in patients with AD.