It is possible that veterinary medicines remain in livestock food products, according to the use of many and
various veterinary medicines to protect against disease when livestock animals are breed in limited space. Concentrated and
continuous monitoring of residues is needed due to increases in resistance to antibiotics and side effects by eating livestock
food products. We developed an analysis method for detecting streptomycin, dihydrostreptomycin, neomycin, gentamicin
and spectinomycin in meat using LC/MS/MS and measured sensitivity, precision, accuracy, linearity and recovery
according to CODEX guidelines to acquire confidence in the analysis method. Based on the results, we acquired good
sensitivity compared to the maximum residue limit (MRL) as limits of detection (LOD) were 0.002-0.016 mg/kg and limits
of quantification (LOQ) were 0.006-0.050 mg/kg. The analysis method satisfied the CODEX guidelines. The linearity (r2)
values of aminoglycoside antibiotics were 0.9936-0.9980, recoveries were 60-110% and relative standard deviations (RSD)
were within 15%. As a result of monitoring for residues in a total 250 samples of livestock foods such as pork, chicken,
and beef by the confirmed method, dihydrostreptomycin and gentamicin were detected in 5 pork samples. The residues of
these antibiotics were within the MRLs. Thus, the detection ratio was 2% as 5 samples were identified from 250 samples.