Fermented compost made from medicinal herb waste (MHWC) as an environment-friendly manure was applied to determine the physicochemical characteristics of Rubus coreanus Miquel (Bokbunja). MHWC, poultry manure compost (PMC), and MHWC+PMC (1:1, w/w) were applied to 2 year-old Bokbunja plants at levels of 0 (UC: untreated control), 20, and 40 Mg/ha, respectively. The physicochemical characteristics of Bokbunja were investigated 15, 20, and 25 days after flowering (DAF). There were no differences between the chemical compositions of the group. However, the total sugar contents of the fruit harvested 15, 20, and 25 DAF from MHWC were increased 0.54∼0.56, 0.46∼0.53, and 1.80∼2.52%, respectively, as compared with UC. The free sugar content in fruit from the MHWC group was higher compared to the other groups. The fructose and glucose contents were increased 0.08∼0.31% and 0.08∼0.18%, respectively. The organic acid content of the fruit was increased in the MHWC and PMC groups, and citric acid constituted 92.67∼93.59% of the total organic acid content. In conclusion, MHWC treatment is a compost source for quality improvement of total sugar, free sugar, and organic acid contents of Bokbunja.