본 연구에서는 메리필드펩타이드 수지가 치환된 테트라아자거대고리 화합물인 1,4,8,11-tetraazacyclotetradecane bonded Merrifield peptide resin(TDM)과 1,4,8,12-tetraazacyclopentadecane bonded Merrifield peptide resin(TPM)을 합성하였다. 합성된 이온교환수지의 이온교환용량을 측정하였고, 또한 여러 농도의 염화암모늄용액에서 마그네슘이온의 분포계수를 측정하였다. 새롭게 합성한 테트라아자거대고리 화합물(TDM, TPM)을 가지고 컬럼크로마토그래피법을 이용하여 마그네슘 동위원소를 분리하고 그 효용성을 논의하였다.Tetraazamacrocyclic ion-exchangers, 1,4,8,11-tetraazacyclotetradecane bonded Merrifield peptide resin(TDM), 1,4,8,12-tetraazacyclopentadecane bonded Merrifield peptide resin(TPM) were prepared, and the ion-exchange capacity of these TDM and TPM were characterized. The distribution coefficients in various conc. of NH4C1 for magnesium with ion exchangers were determined by using the batch method. We found the isotope separation factors on new prepared tetraazamacrocyclic ion-exchangers bonded Merrifield peptide resin(TDM, TPM). The isotope separation of magnesium was determined by using of breakthrough method of column chromatography.