Objectives & Methods : The purpose of this study is to observe the effects of herbal-acupuncture with Artemisiae Vulgaris Folium extract(AaH-HA) at KI_10_(Eumgok) on osteoporosis in ovariectomized(OVX) ddy mice. We carried out several experimental items to analyze the changes in body weight, urine, weight, uterus index, tibial length, the ash bone weight, tibial BMD, serum ALP, serum osteocalcin, serum Ca, and the levels of Ca, P, Ca/P ratio in tibia, and we performed histological and histomorphological analysis as well.
Results : 1. Herbal-acupuncture with AaH-HA at KI_10_ significantly inhibited the reduction phosphorus level in serum in ovariectomized mice.
2. Herbal-acupuncture with AaH-HA at KI_10_ significantly inhibited the reduction creatinine level in serum in ovariectomized mice.
3. Herbal-acupuncture with AaH-HA at KI_10_ significantly inhibited the increase of tibial osteoclast cells in ovariectomized mice.
4. Herbal-acupuncture with AaH-HA at KI_10_ significantly inhibited the reduction of TBV(trabecular bone volume) and TBT(trabecular bone thickness) in ovariectomized mice.
5. Herbal-acupuncture with AaH-HA at KI_10_ significantly inhibited the overgrowth of tibial growth plate length(GPL) in ovariectomized mice.
Conclusions : In conclusion, our study suggested that Herbal-acupuncturing with AaH-HA at KI_10_ can be effective treatment for osteoporosis.