In order to understand the effect of hydrostatic pressure (HP) on Bacillus subtilis isolated from makgeolli, the survival of B. subtilis after HP treatment (400 MPa for 5 min) in various substrates including phosphate buffer, tryptone soya broth at pH 7 and 4, and makgeolli at pH 4 was evaluated depending on bacterial forms (spores and vegetative cells)and adaptation conditions (25℃ for 3 h, or 10℃ for 24 h). Spores were generally resistant to HP (<1 log reduction)regardless of conditions. In contrast, vegetative cells were generally susceptible to HP (up to 3 log reduction-except makgeolli) and were more susceptible after 3 h at 25℃ compared to 24 h at 10℃. In vegetative cells inoculated makgeolli (7 log CFU/mL), the colonies were not detected after 24 h at 10℃. Consequently, B. subtilis in makgeolli easily existed as spores and the spores were resistant to HP. Results demonstrate that HP was more promising in the inactivation of vegetative cells.