This study is to assess the application of SWAT-CUP(Soil and Water Assessment Tool-Calibration Uncertainty Programs) and to extend daily flow data from 8-day interval flow data which has been measured by Korean Ministry of Environment(MOE). Model sensitivity analysis and calibration were performed with sequential uncertainty fitting(SUIF-2), which is one of the programs interfaced with SWAT, in the package SWAT-CUP. The most sensitive parameters were SOL_K.sol, CH_N2.rte, CN2.mgt, SOL_BD.sol, ALPHA_BF.gw, ALPHA_BNK.rte, SOL_AWC.sol, CH_K2.rte, SFTMP.bsn, GW_DELAY.gw. Following the sensitivity analysis, SWAT-CUP calibration was carried out using 8-day interval flow data from January 2008 to December 2010. The results were then assessed based on the visual agreement and simulated flow plots and the performance statistics generated R2 and NSE which are 0.71 and 0.61 respectively. Results of these statistics indicated that there was a good agreement between the observed and simulated flow. To extend daily flow data from 8-day interval flow data, parameters, which were estimated by SWAT-CUP, re-entered for SWAT model. As a result, the observed flow data were found to reflect the trend of simulated flow data. From these results, it is thought that this method could be used to provide daily flow data using 8-day interval flow data.