The major tributaries in Sapgyo-reservoir watershed at Chungcheongnam-do were monitored for flowrate and water quality in order to analyze the characteristics of watershed and to prepare for implementation of total maximum daily load (TMDL). According to the analytical results of flowrate and water quality monitoring data of sixteen tributaries, the tributaries with the value of flowrate over 0.5 ㎥/s were 62.5% among the monitored tributaries and the value of flowrate in the Cheonancheon, Namwoncheon, Shinyangcheon except Gokgyocheon, Muhancheon, Sapgyocheon was relatively greater than the other tributaries. However, 37.5% of the tributaries were exceeded the water quality standards of Sapgyocheon sub-basin (BOD5 5mg/L and/or below) and the concentration of water pollutants regardless of water quality parameters in Cheonancheon, Maegokcheon, Oncheoncheon including Gokgyocheon located in Gokgyocheon catchment were relatively higher than the other tributaries. The tributaries for improving the water quality, according to stream grouping method based on the results of flowrate and water quality monitoring data, were selected. In the Sapgyo-reservoir watershed, the tributaries for improving water quality, which has a large flowrate and a high concentration of water pollutants, were selected at Cheonancheon, Gokgyocheon, Maegokcheon, Namwoncheon, Oncheoncheon. The various water quality improving plans for those tributaries, in accordance with the reduction of point source pollution by population and livestock, should be established and implemented.