Deformed wing virus (DWV)는 꿀벌응애에 의한 2 차 피해로 알려져 있는 질병인 날개의 기형을 유도하 는 바이러스로 알려져 있었으나 바이러스성 질병에 대한 다양한 연구의 결과 꿀벌응애가 많은 바이러스 들을 매개하기는 하지만 직접적인 현상은 꿀벌바이러 스가 야기하는 것으로 밝혀졌다. 이러한 꿀벌바이러스 가 먹이 및 해충에의 전염을 일으킨다는 사실이 보고 된바 있으며 원인모를 증세로 폐사에 이른 서양뒤영 벌(B. terrestris)을 대상으로 진단을 실시한 결과, DWV 꿀벌바이러스가 진단되었으며, 총 711개의 염기 서열이 RT-PCR을 통해 확인되었고 기존의 꿀벌 바 이러스 DWV와 약 96%이상 유사도를 보인다. 이는 국내에서는 처음으로 DWV에 의한 서양뒤영벌(B. terrestris)의 감염을 확인한 결과이다.Deformed wing virus (DWV) of honeybees (Apis mellifera) is closely associated with characteristic wing deformities, abdominal bloating, paralysis, and rapid mortality of emerging adult bees. Here, we report for the first time the occurrence of DWV-infected bumble bees (Bombus terrestris). For the present study, the detection of DWV virus from the adult bumble bee, death adult bumble bee, mail bumble bee, pupa and larva to the infection cycle was investigated in the same colony. Using specific RT-PCR protocols for the detection of DWV followed by sequencing of the PCR products we could demonstrate that the bumble bees were indeed infected with DWV. The virus was detected from diseased insects, and its partial DWV gene was cloned and sequenced. The partial DWV gene encoding the polyprotein is 711-nt of 235 amino acid residues. The deduced nucleotide sequence of the polyprotein partial gene of DWV showed 96.9%, 96.2%, 96.8%, and 96.5% homology to other structure polyprotein partial gene of DWV, respectively. Phylogenetic analysis further conformed that the deduced nucleotide sequence of the polyprotein partial gene of DWV divided to the outside tree. We describe the first time that presence of Deformed wing virus (DWV) from bumble bee (Bombus terrestris) in korea using RT-PCR.