This study was carried out to develop an analysis method for the determination of anionic and nonionic surfactants. The condition for simultaneous analysis of anionic and nonionic surfactants by high-performance liquid chromatography (HPLC) equipped with an evaporated light scattering detector (ELSD) was investigated. Alkyl polyglucoside (APG) and alpha olefin sulfonate (AOS) were analyzed on a C18-boned silica column with a methanol and water gradient condition by HPLC/ELSD. Calibration curves were linear in the range of 100-500 mg/L on APG and 500- 4000 mg/L on AOS, with a coefficient of determination (r2) of 0.9999. The limits of detection (LOD) for APG and AOS were 2.29 and 16.55 mg/L, and the limits of quantification (LOQ) were 7.63 and 55.16 mg/L, respectively. The recoveries of the surfactants were 99.29% on APG and 96.11% on AOS. The established method provided acceptable precision and accuracy. Our methods would be useful for the detection of APG and AOS in dishwashing detergents.