Biogenic volatile organic compounds (BVOC) are emitted from various plant species into atmospheric environment and playing an important role in the formation of photochemical oxidant. We established the enclosure chamber system for measuring BVOC flux in the field. In order to get the quality assurance and quality control data for the enclosure chamber system, some of VOC were tested by PTR-MS (Proton Transfer Reaction Mass Spectrometer). The enclosure chamber system was successfully evaluated in the field. Especially, PTR-MS allows real-time measurements of VOCs in the air with a high sensitivity and fast response time. The response factors and precisions were estimated and the time to reach equilibrium concentration in the chamber was examined. The measured response factor of VOCs was in the range of 2.40~18.31 and the elapsed time in the chamber was examined after approximately 70 min. The recovery of chamber system was higher than 80%.