This study was carried out to compare the medicinal effects of various fruiting body of Ganoderma species and Cordyceps militaris, Phelinus linteus extracts. ?-glucan and polyphenol are useful ingredient in mushrooms and they were known to have antioxidant activity. We analyzed ?-glucan and polyphenol contents of fruiting body of Ganoderma spp., Cordyceps militaris, and Phellinus linteus. Most Ganoderma spp. exhibited ?-glucan contents of 15 to 20%. Cordyceps militalis showed the highest ?-glucan level of 25%. Interestingly, eight strains of Ganoderma spp. was analyzed to have higher contents of ?-glucan than Phelinus linteus. Polyphenol contents was measured after extraction with different solvents. (D.W., 70% EtOH, 80% MeOH) The level of polyphenol in ASI 7020 strain was at maximum in the water extraction and ASI 7086 showed the highest level in the 70% EtOH extraction. The amounts of polyphenol in strain ASI 7113 was at maximum in the 80% MeOH extraction.