Contents
Flavokawain B and C, isolated from the root of Piper methysticum, inhibit melanogenesis in melan-a cells = Piper methysticum의 뿌리로부터 추출한 Flavokawain B와 C가 Melan-a 세포에서 멜라닌 합성에 미치는 영향 / Jong Hyuk Ryu ; Jeong Ah Lee ; Jae Young Ko ; Jae Sung Hwang 1
요약 1
Abstract 1
1. Introduction 2
2. Materials and Methods 3
2.1. Materials 3
2.2. Cell Culture 3
2.3. Cell Viability 3
2.4. Melanin Assay 3
2.5. Extracellular Tyrosinase Activity Assay 3
2.6. Reverse Transcription Polymerase Chain Reaction(RT-PCR) 4
2.7. Western Blot Analysis 4
2.8. Statistical Analysis 4
3. ResuIts 4
3.1. The Effects of FKB and FKC on the Cell Viability 4
3.2. The Effects of FKB and FKC on the Melanin Synthesis 5
3.3. The Effects of FKB and FKC on the Extracellular Tyrosinase Activity 7
3.4. The Effects of FKB and FKC on the Expression of Melanogenesis-related Protein 8
3.5. The Effects of FKB and FKC on the Expression of Melanogenesis-related Gene Expression 8
3.6. The Effects of FKB and FKC on the Phosphorylation of ERK(1/2) and AKT 9
4. Conclusion and Discussion 12
References 13