To measure the size and weight of the fish, we developed an automatic fish size measurement system using a deep neural network, where the YOLO (You Only Look Once)v3 model was used. To detect fish, an IP camera with infrared function was installed over the fish pool to acquire image data and used as input data for the deep neural network. Using the bounding box information generated as a result of detecting the fish and the structure for which the actual length is known, the size of the fish can be obtained. A GUI (Graphical User Interface) program was implemented using LabVIEW and RTSP (Real-Time Streaming protocol). The automatic fish size measurement system shows the results and stores them in a database for future work.