Animal testing is a widely practiced method used to ascertain the safety and effectiveness of cosmetic products. Nonetheless, this approach imposes profound suffering upon sentient beings, and its outcomes often have limited accuracy in predicting human responses. In addition, since animal testing for cosmetic development takes away other lives in order to pursue human beauty, research to replace animal testing must be continuously conducted. Consequently, there is a growing momentum to find alternatives to using laboratory animals, particularly in highly developed nations. Numerous countries, along with the EU(European Union) , have already prohibited the use of lab animals in cosmetic formulation. In alignment with the prevailing global tendency, the prohibition of animal testing is expanding to encompass domestic assessments of cosmetic toxicity. This study focuses on comprehensively analyzing policies, remedial measures, and R&D(research and development) to establish a framework that enhances regulatory standards and the progressive adoption of alternative methods instead of animal testing. The research objectives encompass the following: 1) An analysis of the trajectory of the technological advancements in Animal Replacement Tests for assessing cosmetic toxicity, 2) Exploration of the test guidelines provided by the OECD(Organization for Economic Co-operation and Development) , 3) An investigation into the tendency of Korean Center for the Validation of Alternative Methods. 4) An examination of pertinent domestic laws and guidelines. Because of customary and economical reasons, animal testing for cosmetic safety evaluation should be stopped. This research is expected to significantly contribute to the future development of cosmetics that adhere to rigorous safety standards by accurately assessing cosmetic toxicity.