목차
[표제지 등]=0,1,2
최종연구보고서=1,3,4
제출문=5,7,2
총목차=7,9,2
방사선 조사 지표 개발 및 손상 조직 치료 기술 개발 연구=9,11,2
요약문=11,13,16
SUMMARY=27,29,22
방사선 조사 지표 개발 및 손상 조직 치료 기술 개발 연구=49,51,2
목차=51,53,2
그림목차=53,55,6
표목차=59,61,4
제1장 서론=63,65,2
제2장 국내외 기술개발 현황=65,67,4
제3장 연구개발수행 내용 및 결과=69,71,1
제1절 연구내용 및 방법=69,71,20
제2절 연구 결과=89,91,32
제4장 연구개발목표 달성도 및 대외 기여도=121,123,2
제5장 연구개발결과의 활용계획=123,125,2
제6장 참고문헌=125,127,123
서지정보양식=248,250,2
Table1. Radiation-induced chromosome rearrangement in cases identified by FISH with the DNA probe for chromosome 1, 2 and 4=213,215,1
Table2. Adjusted chromosome rearrangement per cell equivalents induced by radiation in cancer patients identified by FISH with the DNA probe for chromosome 1, 2 and 4=214,216,1
Table3. Radiation-induced chromosome rearrangement identified by FISH with the DNA probe for chromosome 1, 2 and 4;Numbers and frequencies of color junctions, translocations and dicentrics=215,217,1
Table4. Frequency of premature chromosome condensation in human Go lymphocytes after irradiation with 60Co(이미지참조) r-rays(이미지참조)=216,218,1
Table5. Number of Women with Cancer of the Uterine Cervix by Age and Treatment=217,219,1
Table6. Percentage of Women by the Year of Uterine Cervical Cancer Diagnosis and Type of Radiotherapy=217,219,1
Table7. Characteristics of Patients with Cancer of the Uterine Cervix by Treatment=218,220,1
Table8. Woman-years at Risk for Developing a Second Primary Cancer by Time Since Diagnosis and Treatment=219,221,1
Table9. Observed and Expected Second Primary Cancers by Time to Occurrence=219,221,1
Table10. Observed and Expected Numbers of Second Primary Cancers for Patients with Uterine Cervical Cancer by Treatment Modality=220,222,1
Table11. Age distribution of radiation workers by occupation(1997)=221,223,1
Table12. Distribution of radiation workers by dose(1997)=222,224,1
Table13. Dose distribution of radiation workers by age and industry(1997)=223,225,1
Table14. Distribution of radiation workers by duration of work(1984-1997)=224,226,1
Table15. Average exposure for radiation workers by duration of work(1984-1997)=225,227,1
Table16. Average exposure for radiation workers by age and duration of work(1984-1997)=226,228,1
Table17. Total number of aberrant metaphases observed following radiation theapy of various doses for lung cancer patients=227,229,1
Table18. Types and frequences of structural chromosomes aberrations following irradiations of various doses for lung cancer patients=228,230,1
Table19. Major CBC parameters following irradiations of various doses for lung cancer patients=228,230,1
Table20. Immunological parameters of blood following irradiation of various doses for lung cancer patients=229,231,1
Table21. Other blood parameters following irradiations of various dose for lung cancer patients=229,231,1
Table22. Frequency of micronuclei in binucleated human lymphocytes after irradiation with 60Co r-ray(이미지참조)=230,232,1
Table23. Relative biological effectiveness(RBE) of neutron and r-ray(이미지참조) for inducing apoptotic fragments in mouse intestinal crypt cell=231,233,1
Table24. Relative biological effectiveness(RBE) of neutron and r-ray(이미지참조) irradiation for apoptotic fragments in rat intestinal crypt cell=232,234,1
Table25. The relative sensitivity of apoptotic fragment induction in intestinal crypt cells of rat and mouse 6h after irradiation with 60Co(이미지참조) r-ray(이미지참조)=233,235,1
Table26. Relative biological effectiveness(RBE) of neutron and r-ray(이미지참조) irradiation for inducing apoptotic fragments in human lymphocytes=234,236,1
Table27. Recovery test using [3H]-8-OHdG during analysis of urinary 8-OHdG Volume of urine=235,237,1
Table28. Recovery test using U-14C-oh8Gua during analysis of urinary 8-OHGua Volume of urine=236,238,1
Table29. Effect of irradiation on urinary 8-OHdG=237,239,1
Table30. Effect of irradiation on urinary oh8Gua=238,240,1
Table31. Viable dermal fiblroblast vell number of various chitosan sponge 1 week after cell adhesion=239,241,1
Table32. Survival effect of mouse by Oltipraz=240,242,1
Table33. Survival effect of rat hepatic cell by Oltipraz=241,243,1
Table34. Effect of mEH mRNA expression by Oltipraz=242,244,1
Table35. Effect of GST mRNA expression by Oltipraz=243,245,1
Table36. Effect of mouse survival by vitamin C and E=244,246,1
Table37. Effect of mEH, GST expression by vitamin C and E=245,247,1
Table38. Effect of mouse survival by combined treatment of Oltipraz and vitamin C/E=246,248,1
Table39. Effect of mouse survival by combined treatment of Oltipraz and Lipoate=247,249,1
Figure1. Scatter gram of frequency of translocation in lymphocyte irradiated in vivo, compared to regression line of dose-response curve for lymphocytes irradiated in vitro=135,137,1
Figure2. Scatter gram of frequency of dicentrics in lymphocyte irradiated in vivo, compared to regression line of dose-response curve for lymphocytes irradiated in vitro=136,138,1
Figure3. Dose-response curve of translocation induced by low dose radiation=137,139,1
Figure4. Dose-response curve of dicentrics induced by low dose radiation=138,140,1
Figure5. Premature chromosome condensation of human peripheral lymphocytes fused with CHO cell after r-rays(이미지참조) irradiation=139,141,1
Figure6. Collective dose distribution and radiation-related workers per year=140,142,1
Figure7. Collective dose distribution and cumulative dose of the irradiated workers above 20 mSv per year=141,143,1
Figure8. Dose-response curve of micronuclei in binucleated human lymphocytes after r-rays(이미지참조) irradiation=142,144,1
Figure9. Microscopic findings of typical apoptotic fragments in intestinal crypt cells postirradiation=143,145,1
Figure10. Microscopic findings of typical apoptotic fragments in mouse intestinal crypt cells postirradiation=144,146,1
Figure11. Microscopic findings of typical apoptotic fragments in mouse intestinal crypt cells postirradiation=145,147,1
Figure12. Kinetics of radiation-induced apoptosis in mouse small intestinal crypt cells with 60Co(이미지참조) r-ray(이미지참조) and neutron=146,148,1
Figure13. The dose-response relationship of apoptotic fragment induction in mouse intestinal crypt cells 6h after irradiation with 60Co(이미지참조) r-ray(이미지참조) and neutron=147,149,1
Figure14. Kinetics of gamma radiation-induced apoptosis in rat small intestinal crypt cells in 60Co(이미지참조) r-ray(이미지참조) and neutron=148,150,1
Figure15. The dose-response relationship of apoptotic fragment induction in rat intestinal crypt cells 6h after irradiation with 60Co(이미지참조) r-ray(이미지참조) and neutron=149,151,1
Figure16. Kinetics of gamma radiation-induced apoptosis in lymphocytes and crypt cells of rat=150,152,1
Figure17. Comparison of the dose-response relationship of apoptotic fragment induction in lymphocytes and intestinal crypt cells after irradiation with 60Co(이미지참조) r-ray(이미지참조)=151,153,1
Figure18. Kinetics of gamma radiation-induced apoptosis in mouse small intestinal crypt cells=152,154,1
Figure19. The dose-response relationship of apoptotic fragment induction in mouse and rat intestinal crypt cells 6h after irradiation with 60Co(이미지참조) r-ray(이미지참조) or neutron=153,155,1
Figure20. Comparison of the dose-response relationship of apoptotic fragment induction in crypt cells of mouse and rat 6h after irradiation with 60Co(이미지참조) r-ray(이미지참조)=154,156,1
Figure21. Apoptosis in irradiated human peripheral lymphocytes=155,157,1
Figure22. SEM image of irradiated human peripheral lymphocytes=156,158,1
Figure23. The dose-response relationship of apoptotic fragment induction in human lymphocytes 24, 48 and 72h after irradiation with 60Co(이미지참조) r-ray(이미지참조)=157,159,1
Figure24. The dose-response relationship of apoptotic fragment induction in human lymphocytes 24h after irradiation with 60Co(이미지참조) r-ray(이미지참조) and neutron=158,160,1
Figure25. Expression of radiation-induced epithelial growth factor receptor=159,161,1
Figure26. Expression of radiation-induced c-jun=160,162,1
Figure27. Expression of radiation-induced phosphor-ERK by Western blot analysis=161,163,1
Figure28. Expression of radiation-induced phosphor-Rb by Western blot analysis=162,164,1
Figure29. Dose point kernel for photons=163,165,1
Figure30. Dose distribution map=164,166,1
Figure31. The dose kernel of Re-188=165,167,1
Figure32. The Dose kernel of I-131(606 keV)=166,168,1
Figure33. The dose kernel as a function of distance r=167,169,1
Figure34. Torso phantom=168,170,1
Figure35. Phantom images=169,171,1
Figure36. Dose distribution(tissue)=170,172,1
Figure37. Dose distribution(bone)=171,173,1
Figure38. Dose distribution(lung)=172,174,1
Figure39. Total dose distribution=173,175,1
Figure40. Irradiation of E.coli PQ37 in broth media=174,176,1
Figure41. Irradiation of immobilized E.coli PQ37=175,177,1
Figure42. Irradiation of freeze-dried powder of E.coli PQ37=176,178,1
Figure43. Irradiation of immobilized E.coli PQ37=177,179,1
Figure44. Irradiation of freeze-dried powder of E.coli PQ37=178,180,1
Figure45. SOS induction of capsule according to X-gal and reaction time=179,181,1
Figure46. SOS induction according to staying time of capsule in intestine=180,182,1
Figure47. SOS induction according to storage time of patch=181,183,1
Figure48. SOS induction according to dose of ingested capsule in rabbit=182,184,1
Figure49. Expression of c-fos and c-jun according to time-course by 5Gy in K-562 cells. The lower panels show the autoradiogram of the same filter rehybridized with a probe specific for GAPDH=183,185,1
Figure50. Expression of c-fos and c-jun 3h according to time-course by 5Gy in K-562 cells. The lower panels show the autoradiogram of the same filter rehybridized with a probe specific for GAPDH=183,185,1
Figure51. Ratio of c-fos expression at 3hr according to dose in K-562 cells=183,185,1
Figure52. Ratio of c-jun expression at 3hr according to dose in K-562 cells=183,185,1
Figure53. Expression of c-fos and c-jun 3h according to time-course by 5Gy in U-937 cells. The lower panels show the autoradiogram of the same filter rehybridized with a probe specific for GAPDH=184,186,1
Figure54. Expression of c-fos and c-jun 1h according to time-course by 5Gy in U-937 cells. The lower panels show the autoradiogram of the same filter rehybridized with a probe specific for GAPDH=184,186,1
Figure55. Ratio of c-fos expression at 3hr according to dose in K-562 cells=184,186,1
Figure56. Ratio of c-jun expression at 3hr according to dose in K-562 cells=184,186,1
Figure57. Expression of c-fos and c-jun according to time-course by 5Gy in WI-38 cells. The lower panels show the autoradiogram of the same filter rehybridized with a probe specific for GAPDH=185,187,1
Figure58. Expression of c-fos and c-jun according to time-course by 5Gy in MRC-5 cells. The lower panels show the autoradiogram of the same filter rehybridized with a probe specific for GAPDH=185,187,1
Figure59. Light microscopical findings of manufactured chitosan and artificial dermal skin like sponge=186,188,1
Figure60. Light microscopical findings of chitosan sponge sticked human fibroblast as artificial dermal skin=186,188,1
Figure61. Light microscopical findings of neutralized chitosan sponge with adhesion of human fibroblast as artificial dermal skin whether it contain bFGF or not=187,189,1
Figure62. Histological findings of human dermal fibroblast incubated on chitosan and chitosan/collagen sponge as artificial dermal skin during 4weeks in vitro=187,189,1
Figure63. Transplantation of chitosan sponge in wound portion made by whole removed skin in Balb/c mouse=188,190,1
Figure64. Histological findings of artificial skin from the dorsal part of back in Balb/c mouse 10days after transplantation=188,190,1
Figure65. Histological findings of artificial skin from the dorsal part of back in Balb/c mouse 4weeks after transplantation=189,191,1
Figure66. Cytotoxicity of GBE in human peripheral blood lymphocytes=190,192,1
Figure67. Effects of IR on apoptosis in HPBL=191,193,1
Figure68. Effects of GBE on apoptosis by IR in HPBL=192,194,1
Figure69. GBE effect on micronuclei formation following IR in HPBL=193,195,1
Figure70. Apoptosis by IR in rat HPBL=194,196,1
Figure71. Effects of GBE on Apoptosis in Rat PBL=195,197,1
Figure72. Apoptosis by IR in rat spleen cells=196,198,1
Figure73. Effects of GBE on apoptosis by IR in rat spleen cells=197,199,1
Figure74. Apoptosis by IR in rat intestinal crypt cells=198,200,1
Figure75. Apoptosis by IR in rat intestinal crypt cells=199,201,1
Figure76. GBE effects on apoptosis following IR in rat intestinal crypt cells=200,202,1
Figure77. Effects of GBE on spleen damage by IR in Balb/c mice=201,203,1
Figure78. Changes of MAP kinase by Oltipraz=202,204,1
Figure79. Changes of P450 2E1 expression by exposure of radiation=203,205,1
Figure80. Changes of P450 2E1 expression by exposure of radiation=204,206,1
Figure81. Mitochondria damage by exposure of radiation=205,207,1
Figure82. Mitochondria damage by exposure of radiation=206,208,1
Figure83. ROS production by exposure of radiation=207,209,1
Figure84. ROS production by exposure of radiation=208,210,1
Figure85. Changes of plasma ALT by exposure of radiation=209,211,1
Figure86. Changes of plasma ALT by exposure of radiation=210,212,1
Figure87. Changes of blood glucose by exposure of radiation=211,213,1
Figure88. Changes of blood glucose by exposure of radiation=212,214,1