표제지
목차
요약문 5
SUMMARY 6
제1장 연구개발과제의 개요 9
제1절 연구개발의 목적 9
제2장 국내외 기술개발 현황 11
제1절 국내ㆍ외 기술개발현황 11
1. 국내 11
2. 국외 11
제2절 연구결과가 국내ㆍ외 기술개발 현황에서 차지하는 위치 12
1. 국내 12
2. 국외 13
제3장 연구개발 수행내용 및 결과 14
제1절 연구개발 수행내용 및 범위 14
1. 미역 14
2. 다시마 18
3. 용다시마 22
4. 위탁연구과제 29
제2절 연구결과 30
1. 미역 30
2. 다시마 43
3. 용다시마 53
4. 위탁연구과제 73
제4장 목표달성도 및 관련분야에의 기여도 78
제5장 연구개발결과의 활용계획 82
제6장 참고문헌 83
제7장 부록 87
판권기 89
Table 1. Genetic resources collection of Undaria pinnatifida in 2017-2018 31
Table 2. Development of micro-sporophyte by culture temperature after stimulation of high temperature (27℃ ) 33
Table 3. Development of micro-sporophyte by culture temperature after stimulation of high temperature (30℃ ) 34
Table 4. Relative growth rates of Undaria pinnatifida male and female gametophyte cultured for 20 days under various temperature, irradiance and photoperiod 36
Table 5. Gametophytic maturation and oogonia or spermatia formation of gametophyte under temperature, irradiance and photoperiod conditions in Undaria pinnatifida 37
Table 6. Growth and growth rate of male and female gametophytes of Undaria pinnatifida (Mean value) 38
Table 7. Oogonia formation of female gametophyte under different Nitrate concentration in Undaria pinnatifida 39
Table 8. Micro-sporophytes formation of female gametophyte under different Nitrate concentration in Undaria pinnatifida 39
Table 9. Contract status of nob-exclusive license 42
Table 10. Growth and growth rate of male and female gametophytes of Saccharina japonica (Mean value) 45
Table 11. Gametophytic maturation and oogonia or spermatia formation of gametophyte under temperature conditions of Saccharina japonica 3 strains for 17 days culture 46
Table 12. Gametophytic maturation and micro-sporophyte formation of gametophyte under temperature conditions of Saccharina japonica 3 strains for 17 days culture 46
Table 13. Maturity rate according to the light quality of the gametophytes of Saccharina japonica after 24 days culture 47
Table 14. Oogonia formation of female gametophyte under different Nitrate concentration in Saccharina japonica 48
Table 15. Micro-sporophytes formation of female gametophyte under different Nitrate concentration in Saccharina japonica 48
Table 16. Genetic resources collection of Saccharina sculpera in 2019 53
Table 17. Relative growth rate of free-living gametophytes in Saccharina sculpera according to different temperature, irradiance and photoperiod conditions 56
Table 18. Gametophytic maturation and oogonia or spermatia formation of gametophyte under temperature, irradiance and photoperiod conditions of Saccharina sculpera 57
Table 19. Gametophytic maturation, micro-sporophyte and oogonia or spermatia formation of gametophyte under varius temperature conditions of Saccharina sculpera 58
Table 20. Formation of micro-sporophytes of Saccharina sculpera by culture medium 59
Table 21. Elapsed period until juvenile blade formation according to nursery transplantation date 60
Table 22. Water temperature by depth in Saccharina sculpera cultivation area 61
Table 23. Concentration of dissolved inorganic nutrient in Saccharina sculpera cultivation area 62
Table 24. Monthly maturation of Saccharina sculpera sporophyte (sorus formation) according to transplant depth 63
Table 25. Monthly maturation of Saccharina sculpera sporophytes (sorus formation) according to transplant depth 65
Table 26. Formation of micro-sporophytes from the cross-breeding conditions between Saccharina japonica and Saccharina sculpera 65
Table 27. Length of micro-sporophytes from the cross-breeding conditions between Saccharina japonica and Saccharina sculpera 66
Table 28. List of dominant species by water depth in Sageunjin subtidal zone, Gangneung 67
Table 29. Results of economic analysis of Saccharina sculpera (Apply twice the unit price of Saccharina japonica) 70
Table 30. Physical properties of dried Saccharina japonica and Saccharina sculpera 70
Table 31. Contents of general (nutritional) components of Saccharina sculpera 72
Table 32. Contents of composition amino acids of Saccharina sculpera 72
Table 33. Multiplex PCR condiditions 74
Table 34. SSR detection kit analysis result guide list 74
Fig. 1. Morphometric measurements of Undaria pinnatifida (NIFS, 2011) 17
Fig. 2. Morphometric measurements of Saccharina japonica (NIFS, 2013) 21
Fig. 3. Procedure of the isolation and culture of free-living gametophytes of Undaria pinnatifida. A , Mature sporophyte; B, A cross-section of sporangia; C, Germling after... 35
Fig. 4. Relative growth rate of female (A) and male (B) gametophytic fragments in Undaria pinnatifida according to the initial length of fragment after 20 days culture... 35
Fig. 5. Relative growth rate of female (A) and male (B) gametophytic fragments in Undaria pinnatifida according to the number of initial cells of fragment after 20 days... 36
Fig. 6. Micro-sporophytes density by female and male gametophyte ratio of Undaria pinnatifida 38
Fig. 7. Density of Undaria pinnatifida young sporophytes by date (A) and water depth (B) of nursery transplantation 40
Fig. 8. Seeds produced of Undaria pinnatifida in land. A, primary seed production; B, secondary seed production 40
Fig. 9. Field cultivation of Undaria pinnatifida seeds produced in land. A, primary field cultivation; B, secondary field cultivation 41
Fig. 10. Morphology of new variety Sugwawon-205 (left) and notice of application number for variety production (right) 42
Fig. 11. Length of micro-sporophytes from cross breeding of free-living gametophytes between Saccharina japonica and Saccharina sculpera by culture temperatures. A, 10℃; B, 15℃ 44
Fig. 12. Length of micro-sporophytes from cross breeding of free-living gametophytes between Saccharina japonica and Undaria pinnatifida by culture temperatures. A, 10℃; B, 15℃ 44
Fig. 13. Growth of micro-sporophytes according to light quality 47
Fig. 14. Micro-sporophytes density by female and male gametophyte ratio of Saccharina japonica 47
Fig. 15. Growth of Saccharina japonica micro-sporophytes according to concentration of fertilizer 48
Fig. 16. Density of Saccharina japonica young sporophytes by date (A) and water depth (B) of nursery transplantation 49
Fig. 17. Growth of 4 different strains of Saccharina japonica from February to October 2017. A, Blade length; B, Blade width; C, Weight; D, Biomass 50
Fig. 18. Daily seawater temperature and period affected by typhoon (shadow) at culture ground from January to October 2018 in Haenam, Korea 50
Fig. 19. Growth of 5 different strains of Saccharina japonica from February to October 2018. A, Blade length; B, Weight; C, Blade width; D, Biomass 51
Fig. 20. Mean cell area (A) Number of cell layers (B) in a cross-sectioned view of different size groups of a Korean Sugwawon No. 301 and Chinese Huangguan No. 1 strains of... 52
Fig. 21. Cell arrangement of Korean Sugwawon No. 301 and Chinese Huangguan No. 1 strains of Saccharina japonica at the same culture farm in Haenam, Korea. Cross-sections were... 52
Fig. 22. Notice of application number for variety production right of Sugwawon-301 53
Fig. 23. Procedure of the isolation and culture of free-living gametophytes of Saccharina sculpera. A, Mature sporophyte; B, A cross-section of sporangia; C, Germling after zoospore... 54
Fig. 24. Relative growth rate of female (A) and male (B) gametophytic fragments in Saccharina sculpera according to the initial length of the fragment after 20 days culture under 15℃,... 55
Fig. 25. Relative growth rate of female (A) and male (B) gametophytic fragments in Saccharina sculpera according to the number of initial cells in the fragment after 20 days culture... 55
Fig. 26. Micro-sporophytes density (left) and length (right) by female and male gametophyte ratio of Saccharina sculpera 57
Fig. 27. Amount of released zoospores by release induced condition. A, Drying time; B, Osmotic shock; C, Drying temperature; D, Presence or absence of light 59
Fig. 28. Growth of Saccharina sculpera young sporophytes according to temperature and light intensity. A, 20µmol/㎡/sec; B, 40µmol/㎡/sec 60
Fig. 29. Light intensity by depth in Saccharina sculpera cultivation area 61
Fig. 30. Changes in monthly blade length of Saccharina sculpera in relation to depth. Kelp sporophytes were cultured at a range of depth (5~25m) from December 2019 to October... 63
Fig. 31. Growth of Saccharina sculpera by cultivation region. A, Changes in monthly blade length of Saccharina sculpera in each cultivation region; B, Gangneung; C, Seosan; D, Jindo 64
Fig. 32. Growth of sporophytes from cross breeding of free-living gametophytes between Saccharina japonica and Saccharina sculpera [(A, S accharina japonica (♀) × Saccharina sculpera... 66
Fig. 33. Brochure insert image. A, Changes in monthly blade length of Saccharina sculpera in relation to depth.; B, Diagram of cultivation and depth adjustments of Saccharina... 67
Fig. 34. Seed production process using free-living gametophytes. A, Gametophytes culture; B, Collect the gametophytes; C, Blending the gametophytes; D, Immerse the substrate;... 68
Fig. 35. Seed production process using zoospores. A, Blade maturation; B, Release induction of zoospores; C, Zoospores release; D, Check the density of zoospores; E, Induction... 69
Fig. 36. Brand image of Saccharina sculpera 69
Fig. 37. Functional ingredient content (A, Total polyphenol; B, Total flavonoid) and antioxidant activity (C, ABTS radical scavenging) 71
Fig. 38. PCR test example 73
Fig. 39. 12 selected SSR markers 73
Fig. 40. Multiplex PCR combinination capable of amplifying 12 markers 74
Fig. 41. Network analysis of each odject considering the geographical relative distance 75
Fig. 42. Network analysis using default threshold 75
Fig. 43. Network analysis using minimum spawning tree algorithm 76
Fig. 44. Results of Population structure analysis 77