표제지
목차
요약문 4
SUMMARY 5
제1장 연구개발과제의 개요 8
1. 연구개발의 목적 8
제2장 국내외 기술개발 현황 10
1. 국내 10
2. 국외 10
제3장 연구개발 수행내용 및 결과 12
제1절 연구개발 수행내용 및 범위 12
1. 바리류 사육기술 표준화 연구 12
2. 바리류 환경내성 구명 14
3. 바리류 양식방법별 현장적용 연구 14
4. 측정항목별 분석방법 15
5. 바리류 양식방법별 경제성 분석(위탁) 17
제2절 연구결과 및 고찰 17
1. 바리류 사육기술 표준화 연구 17
2. 바리류 환경내성 구명 40
3. 바리류 양식방법별 현장적용 연구 55
4. 바리류 양식방법별 경제성 분석(위탁) 63
제4장 목표달성도 및 관련분야에의 기여도 65
1. 연구개발 목표의 달성도 65
2. 관련분야 기여도 65
제5장 연구개발결과의 활용계획 66
제6장 참고문헌 67
제7장 부록 70
판권기 71
Table 1. Hybridization cases of groupers (Gold Seed Project, 2017) 11
Table 2. Estimated period of hybrid grouper (RGGG) in each water temperature 18
Table 3. Estimated period of hybrid grouper (LGGG) in each water temperature while 90 days 22
Table 4. Feed efficiency of hybrid grouper (LGGG) in each water temperature while 90 days 22
Table 5. Growth of hybrid grouper (LGGG) in each water temperature while 30 days 23
Table 6. Estimated period of hybrid grouper (LGGG) in each water temperature while 30 days 23
Table 7. Growth rate and feed efficiency of hybrid grouper (LGGG) in each water temperature while 30 days 23
Table 8. Estimated period of hybrid grouper (RGGG) in each feed composition 24
Table 9. Feed efficiency of hybrid grouper (RGGG) in each feed composition 24
Table 10. Estimated period of hybrid grouper (LGGG) in each feed composition 27
Table 11. Feed efficiency of hybrid grouper (LGGG) in each feed composition 27
Table 12. Estimated period of hybrid grouper (LGGG)'s small group (initial weight: 200g) in each feed composition 28
Table 13. Growth rate and feed efficiency of hybrid grouper (LGGG)'s small group (initial weight: 200g) in each feed composition 28
Table 14. Estimated period of hybrid grouper (LGGG)'s large group (initial weight: 1kg) in each feed composition 29
Table 15. Growth rate and feed efficiency of hybrid grouper (LGGG)'s large group (initial weight: 1kg) in each feed composition 29
Table 16. Growth rate of hybrid grouper (RGGG) in each rearing density while 60 days 31
Table 17. Growth rate and feed efficiency of hybrid grouper (LGGG) in each rearing density 33
Table 18. Feed efficiency of hybrid grouper (RGGG) in each feeding rate and number of feeding 37
Table 19. Growth rate and feed efficiency of hybrid grouper (LGGG) in each feeding rate and number of feeding 38
Table 20. Plasma osmolality of hybrid grouper (RGGG) in each salinity 43
Table 21. Growth of hybrid grouper (LGGG) under low-water temperature condition 52
Table 22. Hematological response of hybrid grouper (RGGG) in each concentration of dissolved oxygen 54
Table 23. Growth of hybrid group (RGGG) under sea net cage culture system 55
Table 24. Growth of hybrid group (RGGG) under recirculation and flowing water culture system during 2020 55
Table 25. Survival rate and growth of hybrid groupers (RGGG) during experimental period in sea net cage 56
Table 26. Growth of hybrid groupers (RGGG) under recirculation and flowing water culture system during 2021 56
Table 27. Survival rate and growth of hybrid groupers (LGGG) in each intial size 57
Table 28. Survival rate and growth of hybrid groupers (LGGG) in each methods during experimental period 58
Table 29. Survival rate and growth of hybrid group (LGGG) in each intial size and age 59
Table 30. Growth of hybrid group (LGGG) under aquaculture method 60
Table 31. Survival rate and growth of hybrid group between longtooth grouper and giant grouper (LGGG) and hybrid grouper between red spotted grouper,... 62
Table 32. Economic analysis criteria of hybrid groupers (LGGG and RGGG)'s short-term aquaculture 63
Table 33. Economic analysis criteria of hybrid groupers (LGGG and RGGG)'s intermediate-term aquaculture 63
Table 34. Economic analysis of hybrid groupers (LGGG and RGGG)'s short-term aquaculture 64
Table 35. Economic analysis of hybrid groupers (LGGG and RGGG)'s intermediate-term aquaculture 64
Fig. 1. Morphology of giant grouper, Epinephelus lanceolatus (A) red spotted grouper, E. akaara (B) longtooth grouper, E. bruneus (C) hybrid grouper between... 9
Fig. 2. Breeding aquariums of hybrid groupers (RGGG and LGGG) 12
Fig. 3. Aquaculture method of hybrid groupers (RGGG and LGGG). (A) recirculation, (B) flowing water, (C) sea net cage 15
Fig. 4. Tagged hybrid groupers (RGGG and LGGG) by VIE tag kit 15
Fig. 5. Measurement device of oxygen consumption rate 16
Fig. 6. Growth of hybrid grouper (RGGG) in each temperature. (A) 20℃, (B) 24℃, (C) 28℃, (D), 32℃ 18
Fig. 7. Physiological response of hybrid grouper (RGGG) in each water temperature. (A) cortisol, (B) growth hormone, (C) trypsin 19
Fig. 8. Oxygen consumption rate of hybrid grouper (RGGG) in each water temperature 19
Fig. 9. Histological observation of hybrid grouper (RGGG) in each temperature. (A) 20℃, (B) 24℃, (C) 28℃, (D) 32℃ 20
Fig. 10. Growth of hybrid grouper between longtooth grouper, E. bruneus and giant grouper (LGGG) in each temperature 21
Fig. 11. Growth of hybrid grouper (LGGG) in each temperature. (A) 20℃, (B) 24℃, (C) 28℃, (D) 32℃ 21
Fig. 12. Physiological response of hybrid grouper (RGGG) in each water temperature. (A) cortisol, (B) growth hormone, (C) trypsin 22
Fig. 13. Growth hormone (A) and trypsin (B) concentrations of hybrid grouper (LGGG) in each water temperature 23
Fig. 14. Growth of hybrid (RGGG) in each feed composition. (A) EP, (B) EP+Fish oil, (C) Insect-based EP 24
Fig. 15. Physiological response of hybrid grouper (RGGG) in each feed composition. (A) cortisol, (B) growth hormone, (C) trypsin 25
Fig. 16. Histological observation of hybrid grouper (RGGG)'s midgut epithelium in each feed composition. (A) EP, (B) EP+Fish oil, (C) Insect-based EP 25
Fig. 17. Body weight changes of hybrid (LGGG) in each feed composition 26
Fig. 18. Growth of hybrid (LGGG) in each feed composition. (A) 50/10, (B) 50/20, (C) 50/25, (D) 40/25 26
Fig. 19. Physiological response of hybrid grouper (LGGG) in each feed composition. (A) cortisol, (B) growth hormone, (C) trypsin 27
Fig. 20. Growth hormone (A) and trypsin (B) concentraions of hybrid grouper (LGGG)'s small group (initial weight: 200g) in each feeding composition 28
Fig. 21. Growth hormone (A) and trypsin (B) concentraions of hybrid grouper (LGGG)'s large group (initial weight: 1kg) in each feeding composition 29
Fig. 22. Mortality rate (A), growth (B) and cortisol concentration (C) of hybrid grouper (RGGG) in each rearing density while 30 days 30
Fig. 23. Body weight changes of hybrid (RGGG) in each rearing dentsity while 60 days. (A) 400g (mean value of initial body weight), (B) 800g (mean value of initial... 31
Fig. 24. Physiological response of hybrid grouper (RGGG) in each rearing density. (A~C) small (initial weight: 400g), (D~F) large (inital weight: 800g), (A) (D)... 32
Fig. 25. Growth of hybrid grouper (LGGG) in each rearing density during experimental period (3 months). (A) small (initial weight: 200g), (B) large (inital weight: 1kg) 33
Fig. 26. Physiological response of hybrid grouper (LGGG) in each density during experimental period. (A~C) small (initial weight: 200g), (D~F) large (inital weight: 1kg),... 34
Fig. 27. Body weight changes of hybrid grouper (RGGG) in each number of feeding 35
Fig. 28. Physiological response of hybrid grouper (RGGG) in each number of feeding. (A) cortisol, (B) glucose, (C) GOT, (D) trypsin 36
Fig. 29. Body weight changes of hybrid grouper (RGGG) in each feeding rate while 12 weeks 36
Fig. 30. Physiological response of hybrid grouper (RGGG) in each number of feeding. (A) cortisol, (B) glucose (C) GOT, (D) trypsin 37
Fig. 31. Growth of hybrid grouper (LGGG) in each rate (A) (C) and number (B) (D) of feeding. (A) (B) small (initial weight: 150g), (C) (D) large (initial weight: 1.2kg) 38
Fig. 32. Physiological response of hybrid grouper (LGGG)'s small group (initial weight: 150g) in each rate (A) (B) (C) and number (D) (E) (F) of feeding.... 39
Fig. 33. Physiological response of hybrid grouper (LGGG)'s large group (initial weight: 1.2kg) in each rate (A) (B) (C) and number (D) (E) (F) of feeding.... 40
Fig. 34. Survival rate of hybrid grouper (RGGG) in each environmental factor. (A) water temperature, (B) salinity, (C) ammonia concentration 41
Fig. 35. Oxygen consumption rate of hybrid grouper (RGGG) under low-water temperature condition 42
Fig. 36. Physiological response of hybrid grouper (RGGG)'s liver (up) and kidney (down) under low-water temperature condition. (A, E) cortisol, (B, F) GSH,... 42
Fig. 37. Physiological response of hybrid grouper (RGGG)'s liver (up) and kidney (down) in each salinity. (A, E) cortisol, (B, F) GSH, (C, G) CAT, (D, H) SOD.... 43
Fig. 38. Oxygen consumption rate of hybrid grouper (RGGG) in each salinity 44
Fig. 39. Histological observation of hybrid grouper (RGGG) in each salinity. (A) 5 psu, (B) 25 psu, (C) 34 psu. (a), histological change (giantism) of gill lamella;... 44
Fig. 40. Physiological response of hybrid grouper (RGGG)'s liver (up) and kidney (down) in each concentration of ammonia. (A, E) cortisol, (B, F) GSH,... 45
Fig. 41. Histological observation of hybrid grouper (RGGG)'s liver tissue in each ammonia. (A) 0 ppm (control), (B) 0.5 ppm, (C) 1.0 ppm, (D) 1.5 ppm, (E) 2.0 ppm 46
Fig. 42. Survival rate of hybrid grouper (LGGG) in each environmental factor. (A) water temperature, (B) salinity, (C) ammonia concentration 46
Fig. 43. Oxygen consumption rate of hybrid grouper (LGGG) in each water temperature (A) and salinity (B) 47
Fig. 44. Physiological response of hybrid grouper (LGGG) under low-water temperature condition. (A) cortisol, (B) GSH, (C) CAT, (D) SOD 48
Fig. 45. Histological observation of hybrid grouper (LGGG) in each temperature. Hepatocellular condensation and telangiectasis were observed in 9, 10... 49
Fig. 46. Physiological response of hybrid grouper (LGGG) in each salinity. (A) cortisol, (B) GSH, (C) SOD, (D) CAT, (E) Osmolality 49
Fig. 47. Histological observation of hybrid grouper (LGGG)'s liver A, B, C and gill D, E, F in each salinity. Hepatocellular condensation and telangiectasis were... 50
Fig. 48. Physiological response of hybrid grouper (LGGG) in each concentration of ammonia. (A) cortisol, (B) GSH, (C) SOD, (D) CAT 51
Fig. 49. Survival rate (A), growth (B) and oxygen consumption rate (C) of hybrid grouper (RGGG) under low-water temperature condition 51
Fig. 50. Survival rate of hybrid grouper (LGGG) under low-water temperature condition 52
Fig. 51. Physiological response of hybrid grouper (LGGG) in each water temperature during experimental period. (A) cortisol, (B) SOD, (C) CAT, (D) GSH 53
Fig. 52. Oxygen consumption rate of hybrid grouper (LGGG) under low-water temperature condition 53
Fig. 53. Physiological response of hybrid grouper (RGGG)'s liver (up) and kidney (down) under exposure of each LED illumination and colors. (A, E) cortisol,... 54
Fig. 54. Physiological response of hybrid grouper (RGGG) under aquaculture method. (A) cortisol, (B), growth hormone, (C) trypsin 56
Fig. 55. Physiological response of hybrid grouper (RGGG) in each aquaculture method. (A) cortisol, (B) growth hormone, (C) trypsin 57
Fig. 56. Physiological response of hybrid grouper (LGGG) in each aquaculture method. (A) cortisol, (B) growth hormone, (C) trypsin 58
Fig. 57. Korean rockfish (A) and hybrid grouper (B) in experimental cage under high-water temperature condition 59
Fig. 58. Physiological response of hybrid grouper (LGGG) in sea net cage during high-water temperature season. (A) cortisol, (B) growth hormone, (C) trypsin 60
Fig. 59. Survival rate (A) and physiological response (B) (C) (D) of hybrid group (RGGG) during wintering season (2020) 60
Fig. 60. Underwater filming of hybrid grouper in sea net cages of Tong-young (A) and Geomun-do island (B) 61
Fig. 61. Water temperature change of each experimental site during wintering season. (A) Geomun-do island, (B) Geumo-do island 61
Fig. 62. Physiological response of hybrid groupers (LGGG and RGGG) in sea net cage of Geomun-do island during wintering season. (A) cortisol, (B) growth hormone, (C) trypsin 62