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22대 대한민국 국회 국회정보길라잡이 국회의원검색
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Title Page

Contents

Abstract 9

Introduction 11

Materials and Methods 15

Bacterial Strain and Culture 15

Seaweed Extract Preparation 15

Disc Diffusion Assay 19

Minimum Inhibitory Concentration Assay 21

Checkerboard Microdilution Assay 22

Results 24

Antibacterial activity of Ecklonia cava extract 24

Determination of E. cava Minimum Inhibitory Concentration 27

Antibiotics Resistance Assay 29

Oxytetracycline and Erythromycin MIC Assay 31

Synergism of E. cava EtOAc extract with Oxytetracycline and Erythromycin against S. parauberis 33

Discussion 36

Conclusion 42

References 43

List of Tables

Table 1. Bacterial samples used in this study were isolated from olive... 16

Table 2. Seaweed species used in the study and suspended extract in disc... 17

Table 3. Antibiotics used for antibacterial susceptibility test against... 20

Table 4. Disc diffusion assay of seaweeds extract against Streptococcus parauberis 25

Table 5. Disc diffusion assay of methanol extract and its solvent-soluble extracts from Ecklonia cava... 26

Table 6. Minimum inhibitory concentration (MIC) of the methanol extract and its soluble fractions from... 28

Table 7. Antibiotic resistance assay against Streptococcus parauberis strains 30

Table 8. Minimum inhibitory concentration (MIC) of oxytetracycline and... 32

Table 9. Fractional inhibitory concentration (FIC) index of EtOAc fraction of Ecklonia cava in... 34

Table 10. Fractional inhibitory concentration (FIC) index of EtOAc fraction of Ecklonia cava in... 35

List of Figures

Figure 1. Schematic diagram for solvent-soluble fractionation using... 18

Figure 2. Checkerboard analysis on the combination of Ecklonia cava... 23

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