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Calreticulin (CRT) is a major Ca2+-binding/bufering protein in the lumen of the endopla-smic reticulum. A cDNA encoding CRT, BrCRT1b (Brassica rapa CRT1b), has ben isolated from Chinese cabbage flower bud. Recombinant BrCRT1b21-418 protein showed 45Ca2+-binding and chaperone activity. BrCRT1b transcripts accumulated to high levels in the root, young leaf and flower. Constitutive over-expresion of BrCRT1b promoted robust shoot production and root formation at sub-optimal concentrations of auxin/cytokinin. In spite of enhanced regeneration in tisue culture, the sedling and plant growth rates were slightly inhibited in soil. Thus, our findings sugest that B r C R T 1 b is related to organogenesis during in vitro culture.

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번호 참고문헌 국회도서관 소장유무
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