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Contents
The role of keratinocyte-derived chemokine in hemorrhage-induced acute lung injury in mice / Byoung Hoon Lee ; Tae Jin Lee ; Jae Woo Jung ; Dong Jin Oh ; Jae Chol Choi ; Jong Wook Shin ; In Won Park ; Byoung Whui Choi ; Jae Yeol Kim 1
[요약] 1
Introduction 1
Materials and methods 1
Mice 1
Chemicals and reagents 1
Model of endotoxemia 2
Model of hemorrhagic shock 2
Administration of anti-HMGB1 antibody in hemorrhagic shock 2
Preparation of lung homogenate for ELISA 2
Cytokine ELISA 2
Myeloperoxidase assay 2
Preparation of nuclear extracts from whole lung samples 2
Electrophoretic mobility shift assay 2
Bronchoalveolar lavage 2
Immunohistochemical staining for GRO-alpha 3
Statistical analysis 3
Results 3
Lung neutrophil accumulation as assessed by myeloperoxidase (MPO) activity after hemorrhageor endotoxemia-induced acute lung injury 3
Hemorrhage induces intranuclear translocation of NF-KB(이미지참조), which is blocked by anti-HMGB1 antibody 3
LPS increases of TNF-α, MIP-2 and IL-1β, expressions in the lung 3
Hemorrhage induces a minimal increase of IL-1β expression and no increases of TNF-α or macrophage inflammatory protein (MIP)-2 expression in the lung 4
Hemorrhage induces increased KC expression in the lung 4
Increased expression of KC in lung tissue after hemorrhage confirmed by immunohistochemical staining 4
Hemorrhage induces increased KC expression in bronchoalveolar lavage fluid 4
Hemorrhage induces increased KC plasma expression 5
Discussion 5
REFERENCES 6
초록보기 더보기
Dominant inflammatory cytokines might be different depending on the underlying
causes of acute lung injury (ALI). The role of kertinocyte-derived chemokine (KC),
a potent chemoattractant for neutrophils, has not been clearly established in hemorrhage-
induced ALI. In this study, lung injury and cytokine expressison were evaluated
in LPS- or hemorrhage-induced ALI models of BALB/c mice. The myeloperoxidase
activities at 4 hr after hemorrhage and LPS-injection were 47.4±13.0 and
56.5±16.4 U/g, respectively. NF-κB activity peaked at 4 hr after hemorrhage, which
was suppressed to the control level by anti-high mobility group B1 (HMGB1) antibody.
Lung expressions of TNF-α, MIP-2, and IL-1β were increased by LPS injection.
However, there was only a minimal increase in IL-1β and no expressions of
TNF-αor MIP-2 in hemorrhage-induced ALI. In contrast, lung KC increased significantly
at 4 hr after hemorrhage compared to control levels (83.1±12.3 vs. 14.2±
stained positive for KC. Increased KC was also observed in bronchoalveolar lavage
fluid and plasma. KC plays an important role in hemorrhage-induced ALI.
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