Saccharomycopsis lipolytica의 변이균주의 분리 및 특성 / 조석금 1
ABSTRACT 1
I. 서론 1
II. 재료 및 실험방법 2
1. 사용균주 및 기본배지 2
2. Isocitrate lyase 유도균체의 조제 2
3. 온도감수성 변이균주의 분리 2
4. 온도 shift up 효과의 측정 2
5. 균체 농도와 구연산의 측정 3
6. 효소활성 측정 3
III. 결과 및 고찰 3
1. Isocitrate lyase의 유도 3
2. 온도감수성 변이균주의 분리 및 특성 4
3. 배양온도에 따른 isocitrate lyase의 생성과 비생성 속도 5
4. 온도 shift up의 효과 5
IV. 요약 6
〈참고문헌〉 7
초록보기
Temperature-sensitive revertant could grow on acetic acid at 23℃ but not at 33℃, MX9-11, isolated from mutant deficient in the activity of isocitrate lyase and its properties were investigated. The activity of isocitrare lyase and specific rate of isocitrate lyase synthesis decreased according to increase culture temperature from 23 to 33℃ in acetic acid as carbon source. Arapid cessation of increase enzyme activity observed when the temperarure was shift up from 23 to 33℃ but cell growth was continued. On the other hand, the revertant also exhibited temperature-sensitive in n-hexadecane medium as carbon source, and the amount of isocitric acid was nearly equal produced to that at 23℃ when the temperature shift up from 23 to 33℃.