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초록보기

Background: The purpose of this study was to evaluate recently developed real-time polymerase chain reaction (PCR) assay kit to detect Mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM) in respiratory specimens.

Methods: We assessed the positive rate of the real-time PCR assay to detect MTB and NTM in 87 culture-positive specimens (37 sputum, 50 bronchial washing), which were performed real-time PCR by using Real-QTM MTB&NTM Kit from January 2009 to June 2009, at Gyeongsang University Hospital. To compare the efficacy with the TB-PCR assay, we evaluated 63 culture-positive specimens (19 sputum, 44 bronchial washing) for MTB or NTM, which were performed TB-PCR by using ABSOLUTETM MTB II PCR Kit from March 2008 to August 2008.

Results: Among 87 specimens tested using real-time PCR, MTB and NTM were cultured in 58 and 29, respectively. The positive rate of real-time PCR assay to detect MTB was 71% (22/31) and 92.6% (25/27) in AFB stain-negative and stain-positive specimens. For NTM, the positive rate of real-time PCR was 11.1% (2/18) and 72.7% (8/11) in AFB stain-negative and stain-positive specimens. Among 63 specimens performed using TB-PCR, MTB and NTM were cultured in 46 and 17, respectively. The positive rate of TB-PCR was 61.7% (21/34) and 100% (12/12) in AFB stain-negative and stain-positive specimens. TB-PCR was negative in all NTM-cultured 17 specimens.

Conclusion: TB/NTM real-time PCR assay is useful to differentiate MTB and NTM in AFB stain-positive respiratory specimens and it is as effective in detecting MTB with TB-PCR.

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참고문헌 (22건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Global Burden of Tuberculosis 네이버 미소장
2 Current Situation of Tuberculosis and Its Control in Korea 소장
3 Diagnostic Standards and Classification of Tuberculosis in Adults and Children. This official statement of the American Thoracic Society and the Centers for Disease Control and Prevention was adopted by the ATS Board of Directors, July 1999. This statement was endorsed by the Council of the Infectious Disease Society of America, September 1999. 네이버 미소장
4 Use of polymerase chain reaction for rapid diagnosis of tuberculosis. 네이버 미소장
5 Clinical Utility of Amplified Mycobacterium Tuberculosis Direct Test in the Diagnosis of Pulmonary Tuberculosis 소장
6 Molecular diagnostics in tuberculosis: basis and implications for therapy. 네이버 미소장
7 An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. 네이버 미소장
8 Recovery Rate and Characteristics of Nontuberculous Mycobacterial Isolates in a University Hospital in Korea 소장
9 Recovery Rate of Nontuberculous Mycobacteria from Acid-Fast-Bacilli Smear-Positive Sputum Specimens 소장
10 Clinical Utility of Polymerase Chain Reaction for the Differentiation of Nontuberculous Mycobacteria in Patients with Acid-fast Bacilli Smear-positive Specimens 소장
11 Yu CM, Koh WJ, Ryu YJ, Jeon K, Choi JC, Kang EH,et al. Usefulness of PCR test for M. tuberculosis for the differentiation of pulmonary tuberculosis and nontuberculous mycobacterial lung disease in patients with smear-positive sputum. Tuberc Respir Dis 2004;57:528-34. 미소장
12 Usefulness of PCR Study in AFB Smear Negative Patients on Admission 네이버 미소장
13 Real-time Polymerase Chain Reaction (PCR) in Microbiology 소장
14 Mycobacterium tuberculosis and Nontuberculous Mycobacteria by PCR Assay 소장
15 Evaluation 네이버 미소장
16 Clinical Usefulness of Real-time PCR and Amplicor MTB PCR Assays for Diagnosis of Tuberculosis 소장
17 Detection of Mycobacterium tuberculosis complex Using Real-time Polymerase Chain Reaction 네이버 미소장
18 (종설 : 비결핵 항산균 폐질환의 진단과 치료) 네이버 미소장
19 Evaluation of Roche Amplicor PCR assay for Mycobacterium tuberculosis. 네이버 미소장
20 Bergmann JS, Woods GL. Clinical evaluation of the Roche AMPLICOR PCR Mycobacterium tuberculosis test for detection of M. tuberculosis in respiratory specimens.J Clin Microbiol 1996;34:1083-5. 미소장
21 The Clinical Utility of Polymerase Chain Reaction in the Bronchoalveolar Lavage Fluid for the Detection of Mycobacteria 소장
22 Baek SH, Lee JM, Kang MJ, Son JW, Lee SJ, Kim DG,et al. How reliable is sputum PCR test in the diagnosis of pulmonary tuberculosis when sputum smear is negative? Tuberc Respir Dis 2001;50:222-8. 미소장

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