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Developing novel methanol-feeding strategies for the improved production of heterologous proteins in high cell-density fed-batch cultures of Pichia pastoris has been of great interest during recent years. In this study, a recombinant P. pastoris strain (GS115/His+ Mut+) producing β-galactosidase (β-Gal) was used to investigate conventional feeding strategies and to develop a new strategy to increase the recombinant protein production during fedbatch cultures on methanol. Three types of conventional methanol-feeding strategies, including μ-stat, dissolved oxygen-stat (DO-stat) and constant methanol concentration were investigated and compared with respect to alcohol oxidase (AOX), formate dehydrogenase (FDH) and β-gal activities, and cell dry weight (CDW), methanol, and formaldehyde concentration variations during the production phase. Methanol feeding with μ-stat 0.025/h exhibited the highest β-gal activity. Supplementing ammonium and magnesium in μ-stat 0.025/h did not affect the cell growth or methanol or formaldehyde concentrations throughout the fermentation but did improved the maximum β-gal activity from 148.2 to 158.1 kU/mL. A new three-step methanol-feeding strategy was developed based on the results obtained from conventional feeding strategies, which started with μ-stat 0.025/ h for 5 h, then μ-stat 0.030/h, and finally, was switched to DO-stat when maintaining the DO above 20% air saturation became difficult. Implementation of this new feeding strategy resulted in a CDW of 107.2 ± 0.7 g/L, AOX specific activity of 0.1890 ± 0.0030 U/mg CDW, and β-gal activity of 173.5 ± 2.1 kU/mL after 29 h of fermentation, which shows a 5.6, 29.1, and 15.7% increase in CDW, AOX, and β-gal activity, respectively, compared to that of μ-stat at 0.025/h.

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참고문헌 (34건) : 자료제공( 네이버학술정보 )

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번호 참고문헌 국회도서관 소장유무
1 Cregg, J. M. (2007) Pichia Protocols. 2nd ed. Humana Press Inc., Totowa, NJ, USA. 미소장
2 Lin-Cereghino, J. and J. M. Cregg (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24: 45-66. 미소장
3 Lin-Cereghino, J., P. Geoff, C. Ilgen, and J. M. Cregg (2002) Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr. Opin. Biotechnol. 13: 329-332. 미소장
4 Macauley-Patrick, S., M. L. Fazenda, B. McNeil, and L. M. Harvey (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22: 249-270. 미소장
5 Cos, O., R. Ramón, J. L. Montesinos, and F. Valero (2006) Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review. Microb. Cell Fact. 5: 17. 미소장
6 Hartner, F. S. and A. Glieder (2006) Regulation of methanol utilisation pathway genes in yeasts. Microb. Cell Fact. 5: 39. 미소장
7 Biotechnology: Promethean Science or Obsession? 네이버 미소장
8 Sola, A., P. Jouhten, H. Maaheimo, F. Sanchez-Ferrando, T. Szyperski, and P. Ferrer (2007) Metabolic flux profiling of Pichia pastoris grown on glycerol/methanol mixtures in chemostat cultures at low and high dilution rates. Microbiol. 153: 281-290. 미소장
9 Khalilzadeh, R., S. A. Shojaosadati, N. Maghsoudi, J. Mohammadian- Mosaabadi, M. R. Mohammadi, A. Bahrami, N. Maleksabet, M. A. Nassiri-Khalilli, M. Ebrahimi, and H. Naderimanesh (2004) Process development for production of recombinant human interferon-γ expressed in Escherichia coli. J. Ind. Microbiol. Biotechnol. 31: 63-69. 미소장
10 Biotechnology in the Eastern Bloc 네이버 미소장
11 Biotechnology in Turkey: an overview. 네이버 미소장
12 Biotechnology and bioeconomy in China. 네이버 미소장
13 Lin, J., D. Panigraphy, L. B. Trinh, J. Folkman, and J. Shiloach (2000) Production process for recombinant human angiostatin in Pichia pastori. J. Ind. Microbiol. Biotechnol. 24: 31-35. 미소장
14 Minning, S., A. Serrano, P. Ferrer, C. Sola, R. D. Schmid, and F. Valero (2001) Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. J. Biotechnol. 86: 59- 70. 미소장
15 Biotechnology Journal 10/2010 네이버 미소장
16 Biotechnology Journal 3/2008 네이버 미소장
17 Biotechnological studies in the Far-Eastern Region of Russia. 네이버 미소장
18 Biotechnology: Promethean Science or Obsession? 네이버 미소장
19 Wang, Y., Z. Wang, G. Du, Z. Hua, L. Liu, J. Li, and J. Chen (2009) Enhancement of alkaline polygalacturonate lyase production in recombinant Pichia pastoris according to the ratio of methanol to cell concentration. Bioresour. Technol. 100: 1343- 1349. 미소장
20 Woo, S. H., S. H. Park, H. K. Lim, and K. H. Jung (2005) Extended operation of a pressurized 75-L bioreactor for shLkn-1 production by Pichia pastoris using dissolved oxygen profile control. J. Ind. Microbiol. Biotechnol. 32: 474-480. 미소장
21 Zhang, W., M. A. Bevins, B. A. Plantz, and L. A. Smith (2000) Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotechnol. Bioeng. 70: 1-8. 미소장
22 Biotechnology: What Now? 네이버 미소장
23 Biotechnology Journal 3/2008 네이버 미소장
24 Miller, J. H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, NY, USA. 미소장
25 Keesey, J. (1987) Biochemica Information. 1st ed., Boehringer Mannheim Biochemicals, Indianapolis, IN, USA. 미소장
26 Hopner, T. and J. Knappe (1974) Formate determination with formate dehydrogenase. pp. 1551-1555. In: H. U. Bergmeyer (eds.). Methods of Enzymatic Analysis. Academic Press Inc., NY, USA. 미소장
27 Wagner, L. W., N. H. Matheson, R. F. Heisey, and K. Schneider (1997) Use of a silicone tubing sensor to control methanol concentration during fed-batch fermentation of Pichia pastoris. Biotechnol. Techniques 11: 791-795. 미소장
28 Biotechnology in Turkey: an overview. 네이버 미소장
29 The colorimetric estimation of formaldehyde by means of the Hantzsch reaction. 네이버 미소장
30 Biotechnology and bioeconomy in China. 네이버 미소장
31 Jahic, M., F. Wallberg, M. Bollok, P. Garcia, and S. O. Enfors (2003) Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures. Microb. Cell Fact. 2: 6. 미소장
32 Kuwae, S., M. Ohyama, T. Ohya, H. Ohi, and K. Kobayashi (2005) Production of recombinant human antithrombin by Pichia pastoris. J. Biosci. Bioeng. 99: 264-271. 미소장
33 Biotechnological studies in the Far-Eastern Region of Russia. 네이버 미소장
34 Sinha, J., B. A. Plantz, M. Inan, and M. M. Meagher (2005) Causes of proteolytic degradation of secreted recombinant proteins produced in methylotrophic yeast Pichia pastoris: Case study with recombinant ovine interferon-τ. Biotechnol. Bioeng. 89: 102-112. 미소장

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