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Gram-negative bacterium Haemophilus parasuis has recently become one of the most important etiological agents causing serious systemic disease (Glasser’s disease) in pigs. Antibiotic therapy has played a crucial role in the treatment of this disease. Antibiotic resistance observed from the clinical isolates of this pathogen urges us to discover novel drug targets for antimicrobial agents. In this study, we used a combined strategy including exploration of the gene essentiality and comparison of metabolic pathways to infer drug targets of H.

parasuis. We identified 931 gene products essential for bacterial growth according to the DEG database. One hundred and ninety-nine enzyme-coding genes were found in the genome of H. parasuis but were absent in that of the swine host. Lastly,

we determined 117 enzymes exhibiting essentiality and specificity to H. parasuis as a candidate set of drug targets.

Comparison of metabolic pathways between the pathogen and host showed that 25 targeting enzymes belonged to nine unique pathways of the pathogen. The profile of promising targets identified in our study will provide a useful basis for developing more effective antibiotics against the severe swine disease caused by H. parasuis.

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기사명 저자명 페이지 원문 목차
Expression analysis of lipopolysaccharide-and/or concanavalin A/phorbol myristate acetate-stimulated black rockfish peripheral blood leukocytes using a cDNA microarray to identify up-regulated genes Mun-Gyeong Kwon, Myoung-Ae Park, Jee-Youn Hwang, Gun-Wook Baeck, Jee-Min Jung, Ju-Won Kim, Dae-Won Park, Chan-Il Park pp.111-123

Gene expression profiles analyzed by DNA sequencing of cDNA clones constructed from porcine preadipocytes and adipocytes Dong-Mok Lee, Prati Bajracharya, Eun Jeong Jang, Eun Ju Lee, Sung Hwa Chae, Hong-Seog Park, Se Won Kang, Yong Seok Lee, Ki Ho Lee, Jongsoo Chang … [et al.] pp.125-131

Gene expression profiles during differentiation and transdifferentiation of bovine myogenic satellite cells Eun Ju Lee, Prati Bajracharya, Dong-Mok Lee, Se Won Kang, Yong Seok Lee, Hyun-Jeong Lee, Seong Koo Hong, Jongsoo Chang, Jae Woo Kim, Robert D. Schnabel … [et al.] pp.133-148

Phylogeny and divergence time estimation of Coreoleuciscus splendidus populations(Teleostei: Cypriniformes) endemic to Korea based on complete mitogenome sequences Keun-Yong Kim, Yoon Kwon Nam, In-Chul Bang pp.149-156

Analysis of genetic mapping in a waxy/dent maize RIL population using SSR and SNP markers Kyu Jin Sa, Jong Yeol Park, Kyong-Cheul Park, Ju Kyong Lee pp.157-164

LIPG promoter polymorphism is associated with ischemic stroke in Korean population No Soo Kim, Mi Mi Ko, Min Ho Cha, Ok-Sun Bang pp.165-171

The Drosophila melanogaster retinophilin gene encodes the peripheral membrane protein in photoreceptor cells Sunji Lee, Seong-Baek Lee, Paola Ramirez, Yuree Byun, Joosung Kim, Yongsu Jeong, Kwanghee Baek, Jaeseung Yoon pp.173-178

Evolution and expression analysis of the β-glucosidase(GLU) encoding gene subfamily in maize Li Zhao, Ting Liu, Xia An, Riliang Gu pp.179-187

Characterization of Imcrop, a Mutator-like MITE family in the rice genome Young-Jun Mo, Ki-Young Kim, Woon-Chul Shin, Gun-Mi Lee, Jong-Cheol Ko, Jeong-Kwon Nam, Bo-Kyeong Kim, Jae-Kwon Ko, Yeisoo Yu, Tae-Jin Yang pp.189-198

Cyclooxygenase-2 inhibition by novel Bisaryl imidazolyl imidazole derivatives increases Bax/Bcl-2 ratio and upregulates Caspase-3 gene expression in Caco-2 colorectal cancer cell line Reza Entezari Heravi, Farzin Hadizadeh, Mojtaba Sankian, Jalil Tavakol Afshari, Javad Behravan pp.199-204

Analysis of putative miRNA function using a novel approach, GAPPS-miRTar^GE^ Seung Gu Park, Kyung-Hoon Kwon, Sun Shim Choi pp.205-216

In vitro CpG methylation and garcinol reduce PERV LTR promoter activity Hong-Seok Ha, Young-Choon Lee, Sang-Je Park, Yi-Deun Jung, Kung Ahn, Jae-Woo Moon, Kyudong Han, Keon-Bong Oh, Tae-Hun Kim, Hwan-Hoo Seong … [et al.] pp.217-222

In silico identification of potential drug targets in swine pathogen Haemophilus parasuis Zhuofei Xu, Lifang Ma, Huanchun Chen, Rui Zhou pp.223-230

Cholesterol ester transfer protein gene is associated with high-density lipoprotein cholesterol levels in Korean population Jae Woong Sull, Jong-Eun Lee, Myoungsook Lee, Sun Ha Jee pp.231-235

참고문헌 (39건) : 자료제공( 네이버학술정보 )

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번호 참고문헌 국회도서관 소장유무
1 Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, MillerW and Lipman DJ (1997) Gapped BLAST and PSI-BLAST: anew generation of protein database search programs. Nucl. AcidsRes. 25: 3389-3402. 미소장
2 Akerley BJ, Rubin EJ, Novick VL, Amaya K, Judson N andMekalanos JJ (2002) A genome-scale analysis for identificationof genes required for growth or survival of Haemophilusinfluenzae. Proc. Natl. Acad. Sci. USA 99: 966-971. 미소장
3 Anishetty S, Pulimi M and Pennathur G (2005) Potential drug targetsin Mycobacterium tuberculosis through metabolic pathwayanalysis. Comput. Biol. Chem. 29: 368-378. 미소장
4 Biberstein EL and White DC (1969) A proposal for the establishmentof two new Haemophilus species. J. Med. Microbiol. 2: 75-78. 미소장
5 Brochet M, Couvé E, Zouine M, Poyart C and Glaser P (2008) Anaturally occurring gene amplification leading to sulfonamide andtrimethoprim resistance in Streptococcus agalactiae. J. Bacteriol.190: 672-680. 미소장
6 Butala M, Zgur-Bertok D and Busby SJ (2009) The bacterial LexAtranscriptional repressor. Cell Mol. Life Sci. 66: 82-93. 미소장
7 Cai X, Chen H, Blackall PJ, Yin Z, Wang L, Liu Z and Jin M (2005)Serological characterization of Haemophilus parasuis isolatesfrom China. Vet. Microbiol. 111: 231-236. 미소장
8 Castillo YP and Pérez MA (2008) Bacterial beta-ketoacyl-acyl carrierprotein synthase III (FabH): an attractive target for the designof new broad-spectrum antimicrobial agents. Mini Rev. Med.Chem. 8: 36-45. 미소장
9 Coleman J and Raetz CR (1988) First committed step of lipid A biosynthesisin Escherichia coli: sequence of the lpxA gene. J.Bacteriol. 170: 1268-1274. 미소장
10 El Zoeiby A, Sanschagrin F and Levesque RC (2003) Structure andfunction of the Mur enzymes: development of novel inhibitors.Mol. Microbiol. 47: 1-12. 미소장
11 Enne VI, Livermore DM, Stephens P and Hall LM (2001) Persistenceof sulphonamide resistance in Escherichia coli in the UK despitenational prescribing restriction. Lancet 357: 1325-1328. 미소장
12 Erridge C, Bennett-Guerrero E and Poxton IR (2002) Structure andfunction of lipopolysaccharides. Microbes Infect. 4: 837-851. 미소장
13 Antimicrobial characterization of human beta-defensin 3 derivatives. 네이버 미소장
14 Biotechnolgy: Paper alert 네이버 미소장
15 Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. 네이버 미소장
16 Giaever G, Chu AM, Ni L, Connelly C, Riles L, Véronneau S, DowS, Lucau-Danila A, Anderson K, André B, et al. (2002)Functional profiling of the Saccharomyces cerevisiae genome.Nature 418: 387-391. 미소장
17 Kanehisa M and Goto S (2000) KEGG: kyoto encyclopedia of genesand genomes. Nucl. Acids Res. 28: 27-30. 미소장
18 Knox C, Law V, Jewison T, Liu P, Ly S, Frolkis A, Pon A, BancoK, Mak C, Neveu V, et al. (2011) DrugBank 3.0: a comprehensiveresource for 'omics' research on drugs. Nucl. Acids Res. 39:D1035-1041. 미소장
19 Kollef MH (2008) Broad-spectrum antimicrobials and the treatmentof serious bacterial infections: getting it right up front. Clin.Infect. Dis. 47: S3-S13. 미소장
20 Kotnik M, Anderluh PS and Prezelj A (2007) Development of novelinhibitors targeting intracellular steps of peptidoglycanbiosynthesis. Curr. Pharm. Des. 13: 2283-2309. 미소장
21 Miesel L, Greene J and Black TA (2003) Genetic strategies for antibacterialdrug discovery. Nat. Rev. Genet. 4: 442-456. 미소장
22 Nedbalcova, K, Satran P, Jaglic Z, Ondriasova R, and Kucerova Z(2006) Haemophilus parasuis and Glässer’ disease in pigs: areview. Vet. Med-czech. 51: 168-179. 미소장
23 Structure-based design, synthesis, and study of potent inhibitors of beta-ketoacyl-acyl carrier protein synthase III as potential antimicrobial agents. 네이버 미소장
24 O'Dwyer K, Watts JM, Biswas S, Ambrad J, Barber M, Brulé H,Petit C, Holmes DJ, Zalacain M and Holmes WM (2004)Characterization of Streptococcus pneumoniae TrmD, a tRNAmethyltransferase essential for growth. J. Bacteriol. 186: 2346-2354. 미소장
25 Oliveira S and Pijoan C (2004) Haemophilus parasuis: new trendson diagnosis, epidemiology and control. Vet. Microbiol. 99: 1-12. 미소장
26 Paik J, Kern I, Lurz R and Hakenbeck R (1999) Mutational analysisof the Streptococcus pneumoniae bimodular class A penicillin-binding proteins. J. Bacteriol. 181: 3852-3856. 미소장
27 Plaimas K, Eils R and König R (2010) Identifying essential genesin bacterial metabolic networks with machine learning methods.BMC Syst. Biol. 4: 56. 미소장
28 Projan SJ (2002) New (and not so new) antibacterial targets - fromwhere and when will the novel drugs come? Curr. Opin.Pharmacol. 2: 513-522. 미소장
29 PLoS Pathogens at three years. 네이버 미소장
30 Swine Genome Sequencing Consortium (2010) Pig genome sequence--analysis and publication strategy. BMC Genomics 11:438. 미소장
31 Swarbrick J, Iliades P, Simpson JS and Macreadie I (2008) Folatebiosynthesis - reappraisal of old and novel targets in the searchfor new antimicrobials. Open Enzyme Inhib. J. 1: 12-33. 미소장
32 The COG database: an updated version includes eukaryotes. 네이버 미소장
33 Thanassi JA, Hartman-Neumann SL, Dougherty TJ, Dougherty BAand Pucci MJ (2002) Identification of 113 conserved essentialgenes using a high-throughput gene disruption system inStreptococcus pneumoniae. Nucl. Acids Res. 30: 3152-3162. 미소장
34 Williams AH, Immormino RM, Gewirth DT and Raetz CR (2006)Structure of UDP-N-acetylglucosamine acyltransferase with abound antibacterial pentadecapeptide. Proc. Natl. Acad. Sci. USA103: 10877-10882. 미소장
35 Xu Z, Yue M, Zhou R, Jin Q, Fan Y, Bei W and Chen H (2011)Genomic characterization of Haemophilus parasuis SH0165, ahighly virulent strain of serovar 5 prevalent in China. PLoS One6: e19631. 미소장
36 Zhang R and Lin Y (2009) DEG 5.0, a database of essential genesin both prokaryotes and eukaryotes. Nucl. Acids Res. 37:D455-458. 미소장
37 Zhang YM, White SW and Rock CO (2006) Inhibiting bacterial fattyacid synthesis. J. Biol. Chem. 281: 17541-17544. 미소장
38 Tuberculosis: Diagnostics 네이버 미소장
39 Zhou X, Xu X, Zhao Y, Chen P, Zhang X, Chen H and Cai X (2010)Distribution of antimicrobial resistance among different serovarsof Haemophilus parasuis isolates. Vet. Microbiol. 141: 168-173. 미소장

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