This reserch was performed in order to investigate the anti-inflammatory effects of Bupleuri Radix(BR) on the immune response in vitro. Cellular proliferation and cytokine production were measured in mast cells or mouse B cells or CD4 Th cells. BR water extract inhibited the secretions of TNF-α and IL-6 in PMA/A23187 stimulated HMC-1 cells. It increased proliferation but did not affect the expressions of CD69 or CD23 in rIL-4/anti-CD40 activated B cells. BR reduced surface IgE expression and secreted IgE but increased the production of IL-4, IFN-γ and IgG1 in the same cells. BR caused an increase in proliferation in anti-CD3/anti-CD28 stimulated CD4 Th cells but it did not affect the differentiation of Th1 or Th2 cells. However, IL-2 was increased in BR treated Th2 cells. Considering the above-mentioned results, BR can be applied to a broad range of anti-inflammatory reactions, but our data suggest that it will not be likely to exert any effects on type 1 allergic response.
The Effects of Kakamsihokayongkolmoryeotang on the Hyperlipidemia
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신광식, 권건록, 김재우, 손낙원, 정승현, 신길조, 이원철. BV-2 미교세포를 통한 시호의 신경세포 보호효과에 대한 연구. 정보학회지 10(2):61-69, 2004.
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Stanley, L., Robbins Ramzi, S., Cotran Vinay Kumar. Pathologic Basis of Disease, W.B.Saunders Company, p 40, 1984.
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손의섭, 이영자, 복모란. 간장병, 암 알고 치료받아야 산다. 서울, 북스토리, pp 103-111, 2000.
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中島 泉, 오찬혁 역. 新 면역학 입문. 서울, 지구문화사, p 63, 118, 120, 125, 253, 2000.
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Age-associated increased interleukin-6 gene expression, late-life diseases, and frailty.
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Production of Tumor Necrosis Factor-alpha, Interleukin 1-beta, Interleukin 2, and Interleukin 6 by rat leukocyte subpopulations after exposure to Substance P