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Geldanamycin and its derivatives, inhibitors of heat shock protein 90, are considered potent anticancer drugs, although their biosynthetic pathways have not yet been fully elucidated. The key step of conversion of 4,5-dihydrogeldanamycin to geldanamycin was expected to catalyze by a P450 monooxygenase, Gel16. The adequate bioconversions by cytochrome P450 mostly rely upon its interaction with redox partners. Several ferredoxin and ferredoxin reductases are available in the genome of certain organisms, but only a few suitable partners can operate in full efficiency. In this study, we have expressed cytochrome P450 gel16 in Escherichia coli and performed an in vitro assay using 4,5-dihydrogeldanamycin as a substrate. We demonstrated that the in silico method can be applicable for the efficient mining of convenient endogenous redox partners (9 ferredoxins and 6 ferredoxin reductases) against CYP Gel16 from Streptomyces hygroscopicus. The distances for ligand FDX4-FDR6 were found to be 9.384 Å.

Similarly, the binding energy between Gel16-FDX4 and FDX4-FDR6 were -611.88 kcal/mol and -834.48 kcal/mol, respectively, suggesting the lowest distance and binding energy rather than other redox partners. These findings suggest that the best redox partners of Gel16 could be NADPH → FDR6 → FDX4 → Gel16.

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기사명 저자명 페이지 원문 목차
Characterization of Gel16 as a cytochrome P450 in geldanamycin biosynthesis and in-silico analysis for an endogenous electron transport system Hemraj Rimal, Sang-Cheol Yu, Byeongsan Lee, Young-Soo Hong, Tae-Jin Oh p.44-54

Development of an RNA expression platform controlled by viral internal ribosome entry sites Hae Li Ko, Hyo-Jung Park, Jihye Kim, Ha Kim, Hyewon Youn, Jae-Hwan Nam p.127-140

Efficient development of stable recombinant Chinese hamster ovary (rCHO) cell lines to produce antibodies by using dimethyl sulfoxide (DMSO) in electroporation Juyoung Byun, Sena Yoon, Yunji Jeong, Uitaek Oh, Sujin Cho, Jeongsoo Park, Yongsu Jeong, Kwanghee Baek, Jaeseung Yoon p.55-58

Detailed mode of action of Arabinan-debranching α-L-arabinofuranosidase GH51 from Bacillus velezensis Gyo Won Oh, Yewon Kang, Chang-Yun Choi, So-Yeong Kang, Jung-Hyun Kang, Min-Jae Lee, Nam Soo Han, Tae-Jip Kim p.37-43

Ecklonia cava extract containing dieckol suppresses RANKL-induced osteoclastogenesis via MAP kinase/NF-κB pathway inhibition and heme oxygenase-1 induction Seonyoung Kim, Seok-Seong Kang, Soo-Im Choi, Gun-Hee Kim, Jee-Young Imm p.11-20

Lichen-associated bacterium, a novel bioresource of polyhydroxyalkanoate (PHA) production and simultaneous degradation of naphthalene and anthracene Shamsun Nahar, Min-Hye Jeong, Jae-Seoun Hur p.79-90

The selective inhibitory activity of a fusaricidin derivative on a bloom-forming cyanobacterium, Microcystis sp. So-Ra Ko, Young-Ki Lee, Ankita Srivastava, Seung-Hwan Park, Chi-Yong Ahn, Hee-Mock Oh p.59-65

Anti-aging activity of Lavandula angustifolia extract fermented with Pediococcus pentosaceus DK1 isolated from Diospyros kaki fruit in UVB-irradiated human skin fibroblasts and analysis of principal components Ji Hoon Ha, A Rang Kim, Keon-Soo Lee, Song Hua Xuan, Hee Cheol Kang, Dong Hwan Lee, Mi Yeon Cha, Hye Jin Kim, Mi An, Soo Nam Park p.21-29

A role of YlBud8 in the regulation of cell separation in the yeast Yarrowia lipolytica Yun-Qing Li, Qing-Jie Xue, Yuan-Yuan Yang, Hui Wang, Xiu-Zhen Li p.141-150

Antimicrobial agents that inhibit the outer membrane assembly machines of gram-negative bacteria Umji Choi, Chang-Ro Lee p.1-10

Phenazine and 1-undecene producing Pseudomonas chlororaphis subsp. aurantiaca strain KNU17Pc1 for growth promotion and disease suppression in Korean maize cultivars Setu Bazie Tagele, Hyun Gu Lee, Sang Woo Kim, Youn Su Lee p.66-78

The antimicrobial peptide CopA3 inhibits Clostridium difficile toxin A-induced viability loss and apoptosis in neural cells I Na Yoon, Jae Sam Hwang, Joon Ha Lee, Ho Kim p.30-36

Lipoteichoic acid isolated from Lactobacillus plantarum maintains inflammatory homeostasis through regulation of Th1- and Th2- induced cytokines Ji Eun Ahn, Hangeun Kim, Dae Kyun Chung p.151-159

Simple and novel assay of the host-guest complexation of homocysteine with cucurbit[7]uril Se-Ho Park, Jae-Yeul Lee, Hyun-Nam Cho, Kyoung-Ran Kim, Seun-Ah Yang, Hee-Joon Kim, Kwang-Hwan Jhee p.114-126

Gold nanoparticles conjugation enhances antiacanthamoebic properties of nystatin, fluconazole and amphotericin B Ayaz Anwar, Ruqaiyyah Siddiqui, Muhammad Raza Shah, Naveed Ahmed Khan p.171-177

Immunomodulatory properties of Lactobacillus plantarum NC8 expressing an anti-CD11c single-chain Fv fragment Jing Liu, Guilian Yang, Xing Gao, Zan Zhang, Yang Liu, Xin Yang, Chunwei Shi, Qiong Liu, Yanlong Jiang, Chunfeng Wang p.160-170

Characteristics of bacterial communities in biological filters of full-scale drinking water treatment plants Yonkyu Choi, Yeongseop Cha, Bogsoon Kim p.91-104

Zinc ions affect siderophore production by fungi isolated from the Panax ginseng rhizosphere Khalid Abdallah Hussein, Jin Ho Joo p.105-113

참고문헌 (41건) : 자료제공( 네이버학술정보 )

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번호 참고문헌 국회도서관 소장유무
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