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Comparative primary metabolic and lipidomic profiling of freshwater and marine Synechocystis strains using by GC-MS and nanoESI-MS analyses / YuJin Noh ; Hwanhui Lee ; Seong-Joo Hong ; Hookeun Lee ; Byung-Kwan Cho ; Choul-Gyun Lee ; Hyung-Kyoon Choi 1

Abstract 1

1. Introduction 1

2. Materials and Methods 2

2.1. Culture conditions and sample collection 2

2.2. Measurement of cell growth 2

2.3. Primary metabolic profiling of Synechocystis 6803 and Synechocystis 7338 by GC-MS 2

2.4. Lipidomic profiling of Synechocystis 6803 and Synechocystis 7338 by nanoESI-MS 3

2.5. Data processing and multivariate statistical analyses 3

3. Results and Discussion 3

3.1. Comparison of cell growth 3

3.2. Identification of comprehensive metabolites and intact lipid species by GC-MS and nanoESI-MS analyses 3

3.3. Comparative profiling of comprehensive metabolites and intact lipid species 3

4. Conclusion 10

Electronic Supplementary Material (ESM) 11

References 11

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