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결과 내 검색 동의어 포함
결과 내 검색 동의어 포함

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초록보기

배경: 균혈증은 치사율이 30-40%에 이르며 환자에게 치명적일 수 있다. 타임 랩스 촬영 기술을 기반으로 한 QMAC-dRAST 시스템(QuantaMatrix, Republic of Korea)은 기존 장비보다 신속하게 항균제 감수성 검사(antimicrobial susceptibility testing, AST) 결과를 보고할 수 있다. 본 연구에서는 양성 임상 혈액 배양 검체에 대한 QMAC-dRAST의 성능을 평가하였다.

방법: 총 204개의 균주가 양성 혈액 배양에서 수집되었고 그 중 104개가 그람양성균, 100개가 그람음성균으로, Staphylococcus spp., Enterococcus spp., Enterobacteriaceae, Pseudomonas aeruginosa, 그리고 Acinetobacter spp.가 포함되었다. AST 결과 해석 알고리즘(AST 알고리즘) 개선 전후로 67개의 그람양성균과 72개의 그람음성균을 검사하였고 그 결과를 비교하였다. 최종 검사에서는 37개의 그람양성균과 28개의 그람음성균이 추가되었고, 최종적으로 204개의 균주를 이용하여 QMAC-dRAST와 Vitek 2 사이의 일치율을 분석하였다. QMAC-dRAST와 Vitek 2 사이의 불일치 AST 결과를 해결하기 위해, 미량액체배지희석법을 시행하였다.

결과: 그람양성균 67개와 그람음성균 72개를 이용한 1, 2차 시험에서 AST 알고리즘을 개선한 결과, QMAC-dRAST와 Vitek 2의 categorical agreement가 각각 92.7%, 96.2%에서 94.3%, 96.5%로 증가하였다. 204개의 모든 균주 대상으로는 각각 94.5%, 95.4%의 categorical agreement와, 4.8%, 0.6%의 very major error와, 2.5%, 2.0%의 major error가 관찰되었으며, minor error는 2.1%, 3.0%로 나타났다.

결론: QMAC-dRAST 시스템은 신뢰할 만한 성능과 기존 방법보다 빨리 AST 결과를 보고할 수 있는 장점을 가지고 있다. 이 시스템은 향후 AST 알고리즘을 추가적으로 개선하여 기존 AST 시스템과 더욱 높은 일치율을 보여줄 것이다.

Background: Bacteremia is life-threatening to patients, with a case fatality rate of 30–40%. The QMAC-dRAST system (QuantaMatrix, Republic of Korea), which is based on time-lapse imaging technology, can generate antimicrobial susceptibility testing (AST) results earlier than conventional equipment. Here, we evaluated the performance of QMAC-dRAST for positive blood culture samples.

Methods: In total, 204 isolates were collected from positive blood cultures, with 104 Gram-positive cocci (GPC) and 100 Gram-negative rods (GNR), including Staphylococcus spp., Enterococcus spp., Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter spp. Before and after improvement of the AST algorithm, 67 GPC isolates and 72 GNR isolates were tested and their results were compared. In the final test, 37 GPC and 28 GNR were added, and the agreement rates between QMAC-dRAST and Vitek 2 were analyzed using 204 samples. To resolve discrepant AST results between two systems, broth microdilution tests were performed.

Results: In the first and second tests with 67 GPC and 72 GNR, the categorical agreement (CA) between QMAC-dRAST and Vitek 2 was increased from 92.7% and 96.2% to 94.3% and 96.5% by updating the AST algorithm, respectively. For all 204 samples, the agreement rates were 94.5% and 95.4% for CA; 4.8% and 0.6% for very major errors; 2.5% and 2.0% for major errors; and 2.1% and 3.0% for minor errors.

Conclusions: The QMAC-dRAST system has reliable performance and the advantage of faster AST reporting than conventional methods. This system will demonstrate more acceptable agreement rates with conventional AST systems in the future by improving AST algorithms.

권호기사

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 목차
감염병 체외진단용 의료기기 현황과 전망 : Current status and perspectives for in vitro medical devices for infectious disease diagnosis : survey of product development and clinical trials / 제품 개발 및 임상적 성능시험 설문조사 김수민, 이승준, 김선주 p. 65-71

FLT3-ITD 검사의 국내 현황 조사(2021) = Status of FLT3-ITD analysis in Korea (2021) 김현영, 김인숙, 김미영, 신새암, 이자영 p. 72-77

Performance evaluation of QMAC-dRAST using consecutive positive clinical blood culture samples = 연속적인 임상 혈액 배양 양성 검체를 이용한 QMAC-dRAST의 성능 평가 Heekang Choi, Daewon Kim, Mijung Kwon, Jung-Hyun Byun, Bonghwan Jin, Ki Ho Hong, Hyukmin Lee, Dongeun Yong p. 78-84

환자와 의료기관 종사자에서 QuantiFERON-TB Gold-Plus 양성 패턴과 추적 결과 분석 = Analysis of positive patterns and follow-up results of the QuantiFERON-TB Gold-Plus test in the screening of patients and healthcare workers 신은주, 서종도, 김한아, 허미나, 윤여민, 문희원 p. 85-90

Coffin-Siris syndrome : Coffin-Siris 증후군 : 유전형-표현형 상관관계 군집 분석 및 새로운 변이 / genotype-phenotype clustering and novel variants Joowon Jang, Seung Won Chae, Ho Seop Shin, Sung Im Cho, Boram Kim, Jee-soo Lee, Man Jin Kim, Sung Sup Park, Moon-woo Seong p. 91-96

Various ABO genotyping-phenotyping results for ABO blood group discrepancy : ABO 혈액형 불일치에 대한 유전형과 표현형의 후향적 연구 / a retrospective study Soo Jung Lee, Seungwan Chae, Jaeeun Yoo, Dong Wook Jekarl, Seungok Lee, Myungshin Kim, Yonggoo Kim p. 97-102

(An) algorithm to work-up ABO subgroups presenting as weak B in a real-world laboratory : 약-B형과 같은 ABO 아형의 문제해결을 위한 알고리즘 접근법 : B101/O04-variant 대립유전자를 가진 약-B형 증례 / a case with a weak B phenotype harboring B101/O04-variant alleles Seong-Jun Jeon, Joo-Heon Park, Myung-Geun Shin, Chan Park, Eun Jeong Won, Geon Park p. 103-108

(A) case of T-lymphoblastic leukemia with submicroscopic 1p33 deletion resulting in STIL-TAL1 fusion = 초현미경적 1p33 결실로 인한 STIL-TAL1 융합을 동반한 T림프모구백혈병 1예 Soon Hee Chang p. 109-113

Tenosynovitis of the hand caused by Kosakonia cowanii after being pricked by a beanstalk = 콩줄기에 찔린 후 Kosakonia cowanii에 의해 발생한 손 건초염 Shin Young Yun, Jung Rim Kim, Ki Ho Hong, Dongeun Yong, Hyukmin Lee p. 114-119

(The) first cases of OPHN1 exons 1 and 2 deletion in two X-linked intellectual developmental disorder patients in Korea = 두 명의 X-연관 지적 장애 환자에서 발견된 OPHN1 엑손 1, 2 결실의 국내 첫 증례 Kyoung Bo Kim, Soyoung Lee, Do-Hoon Kim p. 120-123

참고문헌 (25건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
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