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목차 1
꿀풀 추출물과 그 활성 화합물인 카페인산의 Na+/H+ exchanger 1 (NHE1) 발현 조절을 통한 피부 pH 개선 효과에 대한 연구 = Study on skin pH improvement effect through regulation of Na+/H+ exchanger 1 (NHE1) expression of prunella vulgaris extract and its active compound, caffeic acid / 박노준 ; 봉심규 ; 박상아 ; 박기현 ; 고영철 ; 김혜원 ; 김수남 1
요약 1
Abstract 1
1. 서론 2
2. 재료 및 방법 3
2.1. 꿀풀 추출 3
2.2. High Performance Liquid Chromatography(HPLC) 분석 3
2.3. 세포배양 3
2.4. 세포 생존능 측정 3
2.5. RNA 추출, cDNA 합성 및 Real-time PCR 4
2.6. Western Blot 4
2.7. 세포 내/외 pH 측정 4
2.8. 통계처리 4
3. 결과 및 고찰 4
3.1. 꿀풀 추출물의 HPCL 패턴 분석 4
3.2. 꿀풀 추출물과 카페인산의 각질형성세포에서 세포 생존능 분석 5
3.3. 꿀풀 추출물과 카페인산의 각질형성세포에서 NHE1 발현 조절 효과 분석 5
3.4. 꿀풀 추출물과 카페인산의 각질형성세포에서 Filaggrin 발현 조절 효과 분석 6
3.5. 꿀풀 추출물과 카페인산의 세라마이드 생합성 조절 효소(SPT) mRNA 발현 분석 6
3.6. 꿀풀 추출물과 카페인산의 각질형성세포 세포내/세포외 pH 조절 효과 분석 8
4. 결론 8
References 9
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