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Background Adipose tissue-derived microvascular fragments are functional vessel segments derived from arterioles, capillaries, and veins. Microvascular fragments can be used as vascularization units in regenerative medicine and tissue engineering containing microvascular networks. However, the in vivo therapeutic and vascularization properties of human microvascular fragments have not been investigated. Methods In this study, we isolated microvascular fragments, stromal vascular fractions, and mesenchymal stem cells from human lipoaspirate and studied their therapeutic efficacy and in vivo vasculogenic activity in a murine model of hindlimb ischemia. In addition, in vivo angiogenic activity and engraftment of microvascular fragments into blood vessels were measured using Matrigel plug assay. Results Both microvascular fragments and stromal vascular fractions contain not only mesenchymal stem cells but also endothelial progenitor cells. In a Matrigel plug assay, microvascular fragments increased the number of blood vessels containing red blood cells more than mesenchymal stem cells and stromal vascular fractions did. The engraftment of the microvascular fragments transplanted in blood vessels within the Matrigel plug significantly increased compared to the engraftment of mesenchymal stem cells and stromal vascular fractions. Moreover, intramuscular injection of microvascular fragments markedly increased blood flow in the ischemic hindlimbs and alleviated tissue necrosis compared to that of mesenchymal stem cells or stromal vascular fractions. Furthermore, transplanted microvascular fragments formed new blood vessels in ischemic limbs. Conclusions These results suggest that microvascular fragments show improved engraftment efficiency and vasculogenic activity in vivo and are highly useful for treating ischemic diseases and in tissue engineering. Graphical Abstract Adipose tissue-derived microvascular fragments are vascularization units in regenerative medicine and tissue engineering containing microvascular networks. Intramuscular injection of microvascular fragments markedly increased blood flow in the ischemic hindlimbs and alleviated tissue necrosis. The present study suggests that microvascular fragments show improved engraftment efficiency and vasculogenic activity in vivo andare highly useful for treating ischemic diseases and in tissue engineering.

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기사명 저자명 페이지 원문 목차
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Design of chimeric GLP-1A using oligomeric bile acids to utilize transporter-mediated endocytosis for oral delivery Seho Kweon, Jun-Hyuck Lee, Seong-Bin Yang, Seong Jin Park, Laxman Subedi, Jung-Hyun Shim, Seung-Sik Cho, Jeong Uk Choi, Youngro Byun, Jooho Park, Jin Woo Park p. 1-18
Polydopamine (PDA) coatings with endothelial vascular growth factor (VEGF) immobilization inhibiting neointimal formation post zinc (zn) wire implantation in rat aortas Jiayin Fu, Qiongjun Zhu, Zhezhe Chen, Jing Zhao, Shaofei Wu, Meng Zhao, Shihui Xu, Dongwu Lai, Guosheng Fu, Wenbin Zhang p. 1-17
Silver nanoparticles–decorated extracellular matrix graft : fabrication and tendon reconstruction performance Sunfang Chen, Dan Cai, Qi Dong, Gaoxiang Ma, Chennan Xu, Xiaogang Ba, Wei Yuan, Bing Wu, Bin Fang p. 1-19
GelMA-based bioactive hydrogel scaffolds with multiple bone defect repair functions : therapeutic strategies and recent advances Bixia Zhou, Xulei Jiang, Xinxin Zhou, Wuyuan Tan, Hang Luo, Shaorong Lei, Ying Yang p. 1-49
Smart and versatile biomaterials for cutaneous wound healing Minxiong Li, Wenzheng Xia, Yi Min Khoong, Lujia Huang, Xin Huang, Hsin Liang, Yun Zhao, Jiayi Mao, Haijun Yu, Tao Zan p. 1-32
Multifunctional nanozyme-reinforced copper-coordination polymer nanoparticles for drug-resistance bacteria extinction and diabetic wound healing Jiahui Zhao, Tengfei Xu, Jichao Sun, Haitao Yuan, Mengyun Hou, Zhijie Li, Jigang Wang, Zhen Liang p. 1-16
Nanoarchitectured conjugates targeting angiogenesis : investigating heparin-taurocholate acid conjugates (LHT7) as an advanced anti-angiogenic therapy for brain tumor treatment Hyung Shik Kim, Jae Hak Seol, Hae Hyun Hwang, Dong Yun Lee p. 1-13
mRNA nanodelivery systems : targeting strategies and administration routes Mujie Yuan, Zeyu Han, Yan Liang, Yong Sun, Bin He, Wantao Chen, Fan Li p. 1-48
Laser-activatable oxygen self-supplying nanoplatform for effciently overcoming colorectal cancer resistance by enhanced ferroptosis and alleviated hypoxic microenvironment Hao Jiang, Hailong Tian, Zhihan Wang, Bowen Li, Rui Chen, Kangjia Luo, Shuaijun Lu, Edouard C. Nice, Wei Zhang, Canhua Huang, Yuping Zhou, Shaojiang Zheng, Feng Gao p. 1-20
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Antifungal bio-coating of endotracheal tube built by overexpressing the MCP1 gene of Saccharomyces boulardii and employing hydrogel as a “house” to antagonize Candida albicans Yunyun Wei, Jianfeng Qiu, Ziqiang Han, Xuanyi Wang, Hui Zhang, Xinya Hou, Xiangwei Lv, Xiaolong Mao p. 1-16
Controlled release of Clenbuterol from a hydroxyapatite carrier for the treatment of Alzheimer’s disease Yi-Wen Lin, Chih-Hsiang Fang, Ya-Jyun Liang, Ching-Yun Yang, Wei-Ting Kuo, Feng-Huei Lin p. 1-19
Exosome-bearing hydrogels and cardiac tissue regeneration Hassan Amini, Atieh Rezaei Namjoo, Maryam Taghavi Narmi, Narges Mardi, Samaneh Narimani, Ozra Naturi, Nafseh Didar Khosrowshahi, Reza Rahbarghazi, Solmaz Saghebasl, Shahriar Hashemzadeh, Mohammad Nouri p. 1-24
Injectable hydrogel nanoarchitectonics with near-infrared controlled drug delivery for in situ photothermal/endocrine synergistic endometriosis therapy Wei Tian, Chenyu Wang, Ran Chu, Haiyan Ge, Xiao Sun, Mingjiang Li p. 1-16
Delivery of axon guidance gene-targeted siRNA delivery system improves neural stem cell transplantation therapy after spinal cord injury Seong Jun Kim, Wan-Kyu Ko, Gong Ho Han, Daye Lee, Min Jai Cho, Seung Hun Sheen, Seil Sohn p. 1-15
Micro-syringe chip-guided intratumoral administration of lipid nanoparticles for targeted anticancer therapy Jeongrae Kim, Sunejeong Song, Minjun Gwak, Hanhee Cho, Wan Su Yun, Namcheol Hwang, Jinseong Kim, Jun Seo Lee, DongHwee Kim, Hyuncheol Kim, Seong Ik Jeon, Taeil Kim, Kwangmeyung Kim p. 1-24
Time-sequential fibroblast-to-myofibroblast transition in elastin-variable 3D hydrogel environments by collagen networks Nhuan T. Do, Sun Young Lee, Yoon Seo Lee, ChaeHo Shin, Daeho Kim, Tae Geol Lee, Jin Gyeong Son, SeHwa Kim p. 1-15
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GM-CSF augmented the photothermal immunotherapeutic outcome of self-driving gold nanoparticles against a mouse CT-26 colon tumor model Jie Dai, Jianmei Li, Yuqin Zhang, Qian Wen, Yun Lu, Yu Fan, Fancai Zeng, Zhiyong Qian, Yan Zhang, Shaozhi Fu p. 1-17
Mesenchymal stem cell-derived secretomes-enriched alginate/ extracellular matrix hydrogel patch accelerates skin wound healing Jae Won Kwon, Cininta Savitri, Byoungha An, Seung Won Yang, Kwideok Park p. 1-20
Nanoarchitectonics of tannic acid based injectable hydrogel regulate the microglial phenotype to enhance neuroplasticity for poststroke rehabilitation Zongjian Liu, Shulei Zhang, Yuanyuan Ran, Huimin Geng, Fuhai Gao, Guiqin Tian, Zengguo Feng, Jianing Xi, Lin Ye, Wei Su p. 1-21
Reduced graphene oxide coated alginate scaffolds : potential for cardiac patch application Nafseh Baheiraei, Mehdi Razavi, Ramin Ghahremanzadeh p. 1-29
Polymer composite microspheres loading 177Lu radionuclide for Interventional Radioembolization therapy and real-time SPECT imaging of hepatic cancer Liu Xiao, Yuhao Li, Ruiman Geng, Lihong Chen, Peng Yang, Mingyu Li, Xia Luo, Yuchuan Yang, Lin Li, Huawei Cai p. 1-14
Fabrication of a three-dimensional bone marrow niche-like acute myeloid Leukemia disease model by an automated and controlled process using a robotic multicellular bioprinting system Dana M. Alhattab, Ioannis Isaioglou, Salwa Alshehri, Zainab N. Khan, Hepi H. Susapto, Yanyan Li, Yara Marghani, Arwa A. Alghuneim, Rubén Díaz-Rúa, Sherin Abdelrahman ... [et al.] p. 1-28
Precise visualization and ROS-dependent photodynamic therapy of colorectal cancer with a novel mitochondrial viscosity photosensitive fluorescent probe Runsha Xiao, Fan Zheng, Kuo Kang, Lei Xiao, Anyao Bi, Yiting Chen, Qi Zhou, Xueping Feng, Zhikang Chen, Hao Yin, Wei Wang, Zihua Chen, Xiaomiao Cheng, Wenbin Zeng p. 1-12
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Recent advances in surface-coordinated metal-organic framework thin films coatings for biomaterials and medical applications : a review Mohammad Mehdi Sabzehmeidani, Mahmood Kazemzad p. 1-31
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Metal-organic framework-based photodynamic combined immunotherapy against the distant development of triple-negative breast cancer Xiaoyan Liang, Min Mu, Bo Chen, Rangrang Fan, Haifeng Chen, Bingwen Zou, Bo Han, Gang Guo p. 1-18
Photothermal therapy of copper incorporated nanomaterials for biomedicine Rong Wang, Ziwei Huang, Yunxiao Xiao, Tao Huang, Jie Ming p. 1-36
Mild heat treatment in vitro potentiates human adipose stem cells : delayed aging and improved quality for long term culture Chiseon Ryu, Minseo Lee, Jae Young Lee p. 1-17
Phototherapy techniques for the management of musculoskeletal disorders : strategies and recent advances Zhenhe Zhang, Rong Wang, Hang Xue, Samuel Knoedler, Yongtao Geng, Yuheng Liao, Michael Alfertshofer, Adriana C. Panayi, Jie Ming, Bobin Mi, Guohui Liu p. 1-36
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Psoralen synergies with zinc implants to promote bone repair by regulating ZIP4 in rats with bone defect Meijing Liu, Junlong Tan, Shuang Li, Chaoyang Sun, Xiangning Liu, Hongtao Yang, Xiaogang Wang p. 1-17
Recent advances in extracellular vesicle engineering and its applications to regenerative medicine Won-Kyu Rhim, Jun Yong Kim, Seung Yeon Lee, Seung-Gyu Cha, Jeong Min Park, Hyeon Jeong Park, Chun Gwon Park, Dong Keun Han p. 1-31
Fe-curcumin nanozyme-mediated immunosuppression and anti-inflammation in experimental autoimmune uveitis Zhengxuan Jiang, Kun Liang, Xiang Gao, Fan Cao, Guangqi An, Siyu Gui, Weiwei Tang, Liping Du, Liming Tao, Xianwen Wang p. 1-15
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Chromophoric cerium oxide nanoparticle-loaded sucking disk-type strip sensor for optical measurement of glucose in tear fluid Sijin Park, Dong Yeon Nam, HeeJae Jeon, Jae Hoon Han, Dawon Jang, Juil Hwang, YeongSeo Park, YoungGeun Han, Young Bin Choy, Dong Yun Lee p. 1-13
Engineering TGF-β inhibitor-encapsulated macrophage-inspired multi-functional nanoparticles for combination cancer immunotherapy Jaehyun Kim, Minjeong Kim, Seok-Beom Yong, Heesoo Han, Seyoung Kang, Shayan Fakhraei Lahiji, Sangjin Kim, Juhyeong Hong, Yuha Seo, Yong-Hee Kim p. 1-16
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참고문헌 (51건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
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