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In this study, microalgal oil was extracted from Nannochloropsis oceanica cultured in an open raceway pond and converted into biodiesel using a solid acid catalyst. Microalgal oil was extracted from two types of microalgae with and without nitrogen starvation using the KOH-solvent extraction method and the fatty acid content and oil extraction yield from each microalgae were compared. The fatty acid content of N. oceanica was 184.8 mg/g cell under basic conditions, and the oil content increased to 340.1 mg/g under nitrogen starvation conditions. Oil extraction yields were 90.8 and 95.4% in the first extraction, and increased to 97.5 and 98.8% after the second extraction. Microalgal oil extracted by KOH-solvent extraction was yellow in color and had reduced viscosity due to chlorophyll removal. In biodiesel conversion using the catalyst SO4²⁻/HZSM-5, solvent-extracted oil showed a FAME content of 4.8%, while KOH-solvent-extracted oil showed a FAME content of 90.4%. Solid acid catalyst application has been made easier by removal of chlorophyll from microalgal oil. The FAME content increased to 96.6% upon distillation, and the oxidation stability increased to 11.07 h with addition of rapeseed biodiesel and 1,000 ppm butylated hydroxyanisole.
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