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국회도서관 홈으로 정보검색 소장정보 검색

초록보기

본 연구에서는 제주도 양식장에서 사육되고 있는 붉바리 장내에서 균주를 분리·동정하여, 산업적으로 활용할 수 있는 프로바이오틱스의 잠재적 가능성을 평가하였다. 붉바리 장내에서 순수 분리된 129균주를 대상으로 1차적으로 자체적인 간이 항균활성 실험을 통해 clear zone이 10 mm 이상 되는 12균주를 선별하여 내담즙성, pH 농도별 내산성, 항산화 활성, 항생제 내성, API kti을 이용한 생리생화학적 특성 실험에 이용하였다. 각 특성 실험을 통해 probiotics으로 잠재적 가능성을 가진 균으로 선발되어진 미생물은 G1, G3, G15, G21, B1, B2, B3, B5 총 8종이다. 8종 중 B균주 그룹(B1, B2, B3, B5)은 G균주 그룹(G1, G3, G15, G21)보다 유의하게 높은 활성을 나타내었다. 선발되어진 미생물의 16S rRNA 염기서열 분석을 통한 계통 분석을 한 결과를 바탕으로 B1 균주는 L. paracasei B1, B2는 L. lactis B2, B3 균주는 Lb. plantarum B3, B5 균주는 L. lactis subsp. hordniae B5, G1은 B. licheniformis G1, G3는 B. velezensis G3, G15는 B. frigoritolerans G15, G21 균주는 B. pumilus G21로 명명하였다.

This study focused on isolating and identifying strains from the gut of Epinephelus akaara cultivated in aquaculture facilities on Jeju Island. The aim was to evaluate the potential of utilizing these strains as probiotics for industrial applications. A total of 129 strains were isolated from the gut of E. akaara and screened based on their ability to create a clear zone of 10 mm or more in a preliminary antimicrobial activity test. Twelve strains were selected for further analysis, including bile resistance, acid tolerance at different pH levels, antioxidant activity, antibiotic susceptibility, and biochemical characteristics using the API kit. Through these characteristic experiments, eight strains (G1, G3, G15, G21, B1, B2, B3, B5) were identified as having potential as probiotics. Among these, the B group strains (B1, B2, B3, B5) exhibited significantly higher activity compared to the G group strains (G1, G3, G15, G21). Based on the phylogenetic analysis of the 16S rRNA gene sequences of the selected microorganisms, the strains were named as follows: B1 strain as Lactobacillus paracasei B1, B2 strain as Lactococcus lactis B2, B3 strain as Lactobacillus plantarum B3, B5 strain as Lactococcus lactis subsp. hordniae B5, G1 strain as Bacillus licheniformis G1, G3 strain as Bacillus velezensis G3, G15 strain as Brevibacterium frigoritolerans G15, and G21 strain as Bacillus pumilus G21.

권호기사

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 목차
양식 붉바리 장관에서 분리된 미생물의 프로바이오틱 잠재력 평가 = Evaluation of the probiotic potential of microorganisms isolated from the intestinal tract of cultured Epinephelus akaara 문영건, 부문수, 이치훈, 박진국, 허문수 p. 1-14

오크라(Abelmoschus esculentus) 추출물의 항염증 및 보습 효과 연구 = Study on anti-inflammatory and moisturizing effect of extracts from Abelmoschus esculentus 유단희, 이인철 p. 15-23

Synergistic effect of essential oils and enterocin produced by Enterococcus faecalis MSW5 against foodborne pathogens Mansi Shukla, Shilpa Gupte p. 24-36

시판 막걸리의 향미특성 분석 = Analysis of flavor characteristics of commercial makgeolli 윤정아, 권세영, 조성욱, 박은희, 서영호, 김명동 p. 37-43

Isolation, identification and use of bacterial strain Ochrobactrum intermedium PDB-3 for degradation of the pesticide chlorpyrifos Diyorbek Kosimov, Lyudmila Zaynitdinova, Aziza Mavjudova, Muzaffar Muminov, Oybek Shukurov p. 44-54

Antioxidant and melanin-reduction effect of mixed extracts of Bixa orellana, Ammi majus, and Glycyrrhiza glabra Mi Jeong Choi, Yu Ri Kim p. 55-64

Bioprospecting of culturable halophilic bacteria isolated from mediterranean solar saltern for extracellular halotolerant enzymes Ahmed Mohamed Ali, Tahany M.A. Abdel-Rahman, Mohamed G. Farahat p. 76-87

High-quality whole genome sequence of a linezolid-resistant and vancomycin-susceptible Enterococcus faecalis isolate ES-2-1 from a pig stool in South Korea Jun Bong Lee, Nguyen Thi Mai Tho, Se Kye Kim, Jang Won Yoon p. 88-90

Complete genome sequence of Weissella koreensis DMW12 isolated from kimchi, traditional Korean fermented vegetables Do-Won Jeong p. 91-93

Complete genome sequence of Pseudarthrobacter sp. IC2-21, a fluquinconazole-degrading soil bacterium Myoungjoo Riu, Songhwa Kim, Jaekyeong Song p. 94-96

Whole genome sequencing of a methicillin-resistant Staphylococcus aureus sequence type 5 strain SA492 isolated from a patient in Korean Ji Heon Park, Gi Yong Lee, Ji Hyun Lim, Soo-Jin Yang p. 97-98

Complete genome sequences of two quinolone-resistant methicillin-resistant Staphylococcus aureus strains isolated from broilers in Korea Gi Yong Lee, Soo-Jin Yang p. 99-101

Complete genome sequence of Massilia sp. KACC 81254BP reveals a potential for degrading polyhydroxyalkanoates Sihyun An, Gyeongjun Cho, Jae-Hyung Ahn, Hang-Yeon Weon, Dayeon Kim, Young-Joon Ko, Jehyeong Yeon, Joon-hui Chung, Han Suk Choi, Jun Heo p. 102-104

Antioxidant and whitening effects of the mixed extract of Ipomoea nil, Arctostaphylos uva-ursi, and Angelica gigas Nakai Mi Jeong Choi, Yu Ri Kim p. 65-75