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The purpose of this study was to examine the hair growth promotion efficacies of ethanolic extracts of Ribes fasciculatum (RFEE) and Callicarpa japonica (CJEE) in 20 mJ/cm2 UVB-irradiated human dermal papilla cells (hDPCs) by measuring Wnt-3a, Wnt-5a, extracellular-signal-regulated kinase (ERK), Akt, and β-catenin. In the quantitative RT-PCR assay, treatment with 25 μg/mL RFEE and CJEE, and 1 μM MXD resulted in increased expression of Wnt-3a mRNA by 74.4% (p 〈 0.01), 211.4% (p 〈 0.001), and 158.7% (p 〈 0.01), respectively, and increased expression of Wnt-5a mRNA by 53.1% (p 〈 0.001), 253.5% (p 〈 0.001), and 170.2% (p 〈 0.001), respectively, compared to the control. In the western blotting assay, treatment with 25 μg/mL CJEE and 1 μM MXD resulted in increased expression of phosphorylated ERK-1/2 (p-ERK-1/2) protein by 31.9% (p 〈 0.001) and 45.4% (p 〈 0.001), respectively, and increased expression of phosphorylated Akt (p-Akt) protein by 29.9% (p 〈 0.01) and 11.3% (p 〉 0.05), respectively, compared to the control. In the β-catenin assay, treatment with 25 μg/mL RFEE and CJEE, and 1 μM MXD resulted in increased production of β-catenin by 17.5% (p 〉 0.05), 39.2% (p 〈 0.01), and 86.7% (p 〈 0.001), respectively, compared to the control.
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