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The advantages of replicon vectors of RNA viruses include a high ability to stimulate innate immunity and exponential amplification of target mRNA leading to high expression of foreign antigens. The present study aimed to construct a DNA-layered nervous necrosis virus (NNV) replicon vector system in which the capsid protein gene was replaced with a foreign antigen gene and to compare the efficiency of foreign antigen expression between the conventional DNA vaccine vector and the present replicon vector. We presented the first report of a nodavirus DNA replicon-based foreign antigen expression system. Instead of a two-vector system, we devised a one-vector system containing both an NNV RNA-dependent RNA polymerase cassette and a foreign antigen-expressing cassette. This single-vector approach circumvents the issue of low foreign protein expression associated with the low co-transfection efficiency of a two-vector system. Cells transfected with a vector harboring hammerhead ribozyme-fused RNA1 and RNA2 (with the capsid gene ORF replaced with VHSV glycoprotein ORF) exhibited significantly higher transcription of the VHSV glycoprotein gene compared to cells transfected with either a vector without hammerhead ribozyme or a conventional DNA vaccine vector expressing the VHSV glycoprotein. Furthermore, the transcription level of the VHSV glycoprotein in cells transfected with a vector harboring hammerhead ribozyme-fused RNA1 and RNA2 showed a significant increase over time. These results suggest that NNV genome-based DNA replicon vectors have the potential to induce stronger and longer expression of target antigens compared to conventional DNA vaccine vectors.

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Construction of nervous necrosis virus (NNV) genome-based DNA replicon vectors for the delivery of foreign antigens Jeong In Yang, Ki Hong Kim p. 1-8

Evaluation of enzyme-linked immunosorbent assay (ELISA) for detection of olive flounder antibodies to viral hemorrhagic septicemia virus (VHSV, genotype Ⅳa) using two Novirhabdovirus antigens Min-Seok Jang, Myung-Joo Oh, Wi-Sik Kim p. 9-15

Protection against spring viremia carp virus (SVCV) by immunization with chimeric snakehead rhabdovirus expressing SVCV G protein Mariem Bessaid, Kyung Min Lee, Jae Young Kim, Ki Hong Kim p. 17-23

2019년부터 2023년까지 국내에서 분리된 참돔이리도바이러스의 계통 분류 및 항원 결정기 예측 = Phylogenetic analysis and antigenic determinant prediction of red sea bream iridovirus isolated in Korea from 2019 to 2023 김국현, 민준규, 정현도, 김광일 p. 25-36

In vitro antimicrobial properties of Bacillus subtilis KCTC 1326 for fish bacterial disease management Ji-Yoon Park, In-Joo Shin, So-Ri Han, Sung-Hyun Kim, Youhee Kim, Se Ryun Kwon p. 37-48

난배양성(viable but non-culturable; VBNC) Edwardsiella piscicida의 특성 연구 = Characterization of viable but non-culturable (VBNC) Edwardsiella piscicida 김아현, 이윤항, 노형진, 허영웅, 김남은, 김도형 p. 49-60

2022년 하절기 제주도 양식 넙치의 병원성 세균 모니터링 = Monitoring of pathogenic bacteria in cultured olive flounder (Paralichthys olivaceus) of Jeju during the summer of 2022 김예지, 전려진, 이영준, 오영은, 이응준, 정준범 p. 61-69

양식 넙치에서 연쇄구균 혼합백신 접종 후 항체가 변화 조사 = Development of antibodies after combination Streptococcus iniae and Streptococcus parauberis of commercial vaccine injection in olive flounder (Paralichthys olivaceus) in farm 한현자, 이덕찬, 김수진, 김태호, 정선명, 김재휘, 최윤재, 조경용, 조미영 p. 71-78

피부 궤양이 발생한 금붕어(Carassius auratus)에서 분리한 Aeromonas veronii의 특성 및 병원성 분석 = A study on the characteristics and pathogenicity of Aeromonas veronii isolated from infected goldfish (Carassius auratus) 정현기, 김민수, 김석호, 최민순 p. 79-88

Possibility of non-invasive diagnostic method for Kudoa septempunctata using a hyperspectral camera Eung Jun Lee, Lyu Jin Jun, Young Juhn Lee, Yeong Eun Oh, Sung Hyun Kim, Heung-soe Kim, Ye Ji Kim, Joon Bum Jeong p. 89-96

순환여과시스템에서 양식되는 바리과 어류 3종[능성어, (Hyporthodus septemfasciatus), 자바리(Epinephelus moara), 교잡종 대왕자바리(E. moara ♀× E. lanceolatus ♂)]의 감염성 질병 모니터링 = Monitoring of infectious diseases in three grouper species (Hyporthodus septemfasciatus, Epinephelus moara and E. moara ♀× E. lanceolatus ♂) cultured in recirculating aquaculture systems 최희재, 최다연, 조성현, 신제윤, 박종연, 방인철, 강예재 p. 97-110

톨트라주릴 합성유도체, N-(4-(4-Fluorophenoxy)-3-methylphenyl) acetamide 근육 주사에 따른 넙치의 자연살해세포(Natural killer cell) 활성 검사 = Natural killer cell activity of olive flounder Paralichthys olivaceus following intramuscular injection of toltrazuril derivative N-(4-(4-Fluorophenoxy)-3-methylphenyl) acetamide 박상협, 김정의, 도정완, 김아란, 김이경 p. 111-122

넙치(Paralichthys olivaceus) 신장에서 Poly (I:C)의 단기 및 장기적인 면역 효과 = Short and long-term immune effects of poly (I:C) in kidney of olive flounder (Paralichthys olivaceus) 성민재, 박영진 p. 123-132

어류 병원성 세균에 대한 월계수(Laurus nobilis) 잎 유래 sesquiterpene lactone과 수산용 항생제의 병용효과 = Combinational effects of sesquiterpene lactones isolated from bay laurel (Laurus nobilis) leaves with antibiotics against fish pathogenic bacteria 임재웅, 최지석, 투르크 아이만, 이미경, 김도형, 강소영 p. 133-138

EHP (Enterocytozoon hepatopenaei)에 오염된 사육기구에 대한 UV-C와 차아염소산칼슘의 소독 효과 = The disinfection effect of UV-C and calcium hypochlorite to shrimp farm instruments contaminated with EHP (Enterocytozoon hepatopenaei) 류지민, 노을빛, 김보성 p. 139-145

RFLP를 이용한 Gyrodactylus salaris의 internal transcribed spacer(ITS) PCR 위양성 판별 = Determination of false positives in PCR diagnostics based on the internal transcribed spacer (ITS) of Gyrodactylus salaris using RFLP 김민성, 최희정, 정지민, 권문경, 황성돈 p. 147-153

(A) suspicious clinical case of goiter in aquarium-reared blood parrot cichlid (Amphilophus citrinellus × Vieja melanurus) with subsequent regression by Iodide administration Sang-Ho Choi, Jeong-Ho Kim p. 155-158

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