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This study was conducted to investigate whether lysophosphatidic acid (LPA) could improve the development of porcine somatic cell nuclear transfer (SCNT) embryos. Porcine SCNT-derived embryos were cultured in chemically defined polyvinyl alcohol (PVA)-based porcine zygote medium (PZM)-4 without or with LPA, and the development, cell proliferation potential, apoptosis, and expression levels of pluripotent markers were evaluated. LPA significantly increased the rates of cleavage and blastocyst formation compared to those seen in the LPA un-treatment (control) group. The expression levels of embryonic development-related genes (IGF2R, PCNA and CDH1) were higher (p 〈 0.05) in the LPA treatment group than in the control group. LPA significantly increased the numbers of total, inner cell mass and EdU (5-ethynyl-2’-deoxyuridine)-positive cells in porcine SCNT blastocysts compared to those seen in the control group. TUNEL assay showed that LPA significantly reduced the apoptosis rate in porcine SCNT-derived embryos; this was confirmed by decreases (p 〈 0.05) in the expression levels of pro-apoptotic genes, BAX and CASP3, and an increase (p 〈 0.05) in the expression level of the anti-apoptotic gene, BCL2L1. In addition, LPA significantly increased Oct4 expression at the gene and protein levels. Together, our data suggest that LPA improves the quality and development of porcine SCNT-derived embryos by reducing apoptosis and enhancing cell proliferation and pluripotency.

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기사명 저자명 페이지 원문 목차
Transplantation of spermatogonial stem cells in stallions Heejun Jung, Minjung Yoon p. 635-644

Meat quality and safety issues during high temperatures and cutting-edge technologies to mitigate the scenario AMM Nurul Alam, Eun-Yeong Lee, Md Jakir Hossain, Abdul Samad, So-Hee Kim, Young-Hwa Hwang, Seon-Tea Joo p. 645-662

Transcriptome sequencing reveals non-coding RNAs respond to porcine reproductive and respiratory syndrome virus and Haemophilus parasuis co-infection in Kele piglets Jing Zhang, Chunping Zhao, Min Yao, Jing Qi, Ya Tan, Kaizhi Shi, Jing Wang, Sixuan Zhou, Zhixin Li p. 663-681

Estimation of genetic correlations and genomic prediction accuracy for reproductive and carcass traits in Hanwoo cows Md Azizul Haque, Asif Iqbal, Mohammad Zahangir Alam, Yun-Mi Lee, Jae-Jung Ha, Jong-Joo Kim p. 682-701

Weighted single-step genome-wide association study to reveal new candidate genes for productive traits of Landrace pig in Korea Jun Park, Chong-Sam Na p. 702-716

Polymorphism analysis of tri- and tetranucleotide repeat microsatellite markers in Hanwoo cattle Shil Jin, Jeong Il Won, Hyoun Ju Kim, Byoungho Park, Sung Woo Kim, Ui Hyung Kim, Sung-Sik Kang, Hyun-Jeong Lee, Sung Jin Moon, Myung Sun Park, Yong Teak Sim, Sun Sik Jang, Nam Young Kim p. 717-725

Lysophosphatidic acid improves development of porcine somatic cell nuclear transfer embryos Ling Sun, Tao Lin, Jae Eun Lee, So Yeon Kim, Ying Bai, Dong Il Jin p. 726-739

Effect of Sasa quelpaertensis Nakai extract on gut microbiota and production performance in pigs Jongan Lee, Hyeon-Ah Kim, Yong-Jun Kang, Yoo-Kyung Kim, Moon-Cheol Shin p. 740-748

Potential use of dried persimmon (Diospyros kaki) byproducts as feed sources for ruminants Sang Moo Lee, Tabita Dameria Marbun, Eun Joong Kim p. 749-762

Effects of single and complex probiotics in growing-finishing pigs and swine compost Kyeongho Jeon, Minho Song, Jihwan Lee, Hanjin Oh, Dongcheol Song, Seyeon Chang, Jaewoo An, Hyunah Cho, Sehyun Park, Hyeunbum Kim, Jinho Cho p. 763-780

Development of a nucleic acid detection method based on the CRISPR-Cas13 for point-of-care testing of bovine viral diarrhea virus-1b Sungeun Hwang, Wonhee Lee, Yoonseok Lee p. 781-791

Evaluation of the quality characteristics of nitrogen gas-stunned chicken meat and small intestine Muhammad Shahbubul Alam, Dong-Heon Song, Sun-Moon Kang, Inho Hwang, Kuk-Hwan Seol, Soo-Hyun Cho, Jung-Hwan Jeon, Hyoun Wook Kim p. 792-806

Development of semi-dried goat meat jerky using tenderizers considering the preferences of the elderly Shine Htet Aung, Md. Altaf Hossain, Ji-Young Park, Young-Sun Choi, Ki-Chang Nam p. 807-833

Correlation analysis of primal cuts weight, fat contents, and auction prices in Landrace × Yorkshire × Duroc pig carcasses by VCS2000 Youngho Lim, Yunhwan Park, Gwantae Kim, Jaeyoung Kim, Jongtae Seo, Jaesik Lee, Jungseok Choi p. 834-845

(A) study of duck detection using deep neural network based on RetinaNet model in smart farming Jeyoung Lee, Hochul Kang p. 846-858

Complete genome sequence of candidate probiotic Limosilactobacillus fermentum KUFM407 Bogun Kim, Ji Yu Heo, Xiaoyue Xu, Hyunju Lee, Duleepa Pathiraja, Jae-Young Kim, Yi Hyun Choi, In-Geol Choi, Sae Hun Kim p. 859-862

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